丹酚酸B预处理的心脏微血管内皮细胞延迟保护作用研究

Research on the Delayed Protecting Function of Cinnabar Phenic Acid B Pretreatment Cardiac Microvessel Endothelial Cell

关键词:  丹酚酸B预处理  微血管内皮细胞  延迟保护

Key words:  Preconditioned with cinnabar phenic acid B  Microvascular endothelial cell (CMEC)  Delayed protection

摘要:

目的:探讨丹酚酸B通过保护心脏微血管内皮细胞(CMEC)抗心肌缺氧损伤作用机制.方法:基于缺血预适应延迟保护机制,通过缺氧/复氧的CMEC损伤模型,观察丹酚酸B预处理的CMEC保护作用.结果:丹酚酸B预处理与缺氧预处理作用相似,均可提高细胞存活率和SOD活性,降低LDH、NO水平.结论:丹酚酸B预处理可激活CMEC内源性延迟保护机制,发挥了拮抗心肌缺血缺氧损伤效应.

Abstract:

Objective:To research the protective mechanism of the extract of Chinese medicine elan-sen root called cinnabar phenic acid B in treating myocardial ischemic by protecting cardiac microvascular endothelial cell (CMEC).Methods:Based on the mechanism of ischemic preconditioning,preconditioned cinnabar phenic acid B was studied to observe the protective mechanism of CMEC,by the model of anoxic-reoxygenation.Results:The effect of preconditioned cinnabar phenic acid B is similar with that of anoxic preconditioning,which can enhance the survival rate of CMEC and the activity of SOD,and decrease the level of LDH and NO.Conclusion:Preconditioned cinnabar phenic acid B can stimulate the protective effect of ischemic preconditioning for CMEC and exert the effect of treating ischemic and anoxic.