石氏捻转补泻手法对健康小鼠能量代谢相关酶的影响

Influence of Shi’s Rotating Reinforcing and Reducing Method on Correlating Enzymes of Energy Metabolism in Healthy Mice

关键词:  石氏捻转补泻手法  能量代谢  酶  作用机制

Key words:  Shi’s rotating reinforcing and reducing method  energy metabolism  enzyme  mechanism

摘要:

目的: 观察石氏捻转补泻手法对健康小鼠能量代谢相关酶活性的影响。方法: 将昆明种小鼠随机分为5组,穴位局部组织选取足三里周围的骨骼肌,远端选取胃。将组织切片进行酶组化染色,以Pearson法显示琥珀酸脱氢酶,Seligman法显示细胞色素氧化酶,以Niles法显示骨骼肌M-ATP酶,Wachstein-Meisel法显示胃Mg²⁺-ATP酶。结果: 1)石氏捻转补法使琥珀酸脱氢酶、细胞色素氧化酶、ATP酶的积分光度、总面积、面积百分数与对照组相比均显著提高(P<0.05),表明能量代谢相关酶活性增强。2)石氏捻转泻法使琥珀酸脱氢酶、细胞色素氧化酶、ATP酶的积分光度、总面积、面积百分数与对照组相比均显著下降(P<0.05),表明能量代谢相关酶活性减弱。结论: 石氏捻转补泻手法操作后能量代谢水平的变化可能是石氏捻转补泻手法温度效应的作用机制之一。

Abstract:

Objective: To observe the influence of Shi’s rotating reinforcing and reducing method on correlating enzyme activity of energy metabolism in healthy mice. Methods: All Kunming mice were divided into 5 groups. The local tissue of acu-point was skeletal muscle around Zusanli and the distant tissue was stomach. Enzymatic histochemical stain was used to show SDH (succinic dehydrogenase with pearson method), CCO (cytochromeoxidase with seligman method), M-ATP (adenosine triphosphatase with Niles method) and Mg²⁺-ATP with Wachstein-Meisel method.Results: Compared with control group, Shi’s rotating reinforcing method could elevate the integral luminosity, total area, area percentage of SDH, CCO, ATP markedly (P<0.05), showing that the enzymatic activity was strengthened. While the reducing method could lower them (P<0.05), showing that the enzymatic activity associated with energy metabolism was weakened. Conclusion: The changes of energy metabolism after using Shi’s reinforcing and reducing method may be one of the mechanisms oftemperature effect.