中药复方更年乐对更年期大鼠单胺类神经递质的影响

Influence of Chinese decoction Gengnianle on monoamine neurotransmitter in climacteric rats

关键词:  更年乐  更年期综合征  补肾疏肝  调补冲任  单胺类神经递质

Key words:  Gengnianle  climacteric syndrome  tonifying the kidney and smoothing the liver  regulating and tonifying the Chong and Ren Channels  monoamine neurotransmitter

摘要:

[目的]探讨补肾疏肝、调补冲任的中药复方更年乐对更年期大鼠单胺类神经递质的影响。[方法]选用11～15月龄雌性SD大鼠,按照SD大鼠自然更年期模型的要求,将其分为更年期模型组与治疗组,另选3月龄大鼠作为青年对照组,分别给予生理盐水和更年乐煎剂灌胃处理。检测指标采用高效液相色谱-电化学法测定下丘脑单胺类神经递质,包括去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)。[结果]更年乐可使模型组大鼠下丘脑升高的5-HT和5-HIAA含量明显下降(P<0.01～P<0.05),使降低的NE含量回升(P<0.05),升高的5-HT/NE比值降至接近青年组水平(P<0.01)。[结论]中药复方更年乐可以调节紊乱的单胺类神经递质水平,从而改善下丘脑的功能。

Abstract:

[Objective] To study the influence on monoamine neurotransmitter in climacteric rats treated by Chinese decoc-tion Gengnianle with the effect of tonifying the kidney,smooth the liver and regulating and tonifying the Chong and Ren Channels. [Method] Twenty-five rats(age:11～15months)were randomly divided into model group and treatment group. Eight rats(age:3months)consisted of the young rat control group.The decoction was given to the treatment group while normal saline to the other two groups.Monoamine neurotransmitter at hypothalamus including NE,DA,5-HT,5-HIAA were detected by HPLT-electrochemical method. [Result] Gengnianle could decrease 5-HT and 5-HIAA(P<0.01,P<0.05),increase NE(P<0.05)and make the ratio of 5-HT/NE near to that of young rat control group(P<0.01). [Conclusion] Chinese decoction Gengnianle can improve the function of hypothalamus through regulating the level of monoamine neurotransmitter.