活血化瘀软坚散结法对子宫内膜异位症细胞免疫和体液免疫功能影响的实验研究

Experimental research of cellular and humoral immunity in endometriosis (EMS) treated by principle of promoting blood circulation and removing blood stasis, softening hardness and resolving hard masses

关键词:  子宫内膜异位症  活血化瘀  软坚散结  中药妇痛宁  免疫

Key words:  endometriosis (EMS)  promoting blood circulation to dispel blood stasis  softening and resolving hard masses  Futongning  immunolgy

摘要:

[目的]探讨妇痛宁颗粒剂治疗治疗子宫内膜异位症(EMS)的免疫学机制。[方法]采用手术移植法制备大鼠子宫内膜异位症模型。实验共分6组,实验结束检测各组大鼠外周血T细胞亚群、血清免疫球蛋白IgA、IgG、IgM、IgE和补体C₃改变。[结果]模型对照组与空白对照组比较CD₃、CD₄、CD₈有明显变化,各项结果均P<0.01。除丹那唑对CD₄、妇痛宁低剂量组对CD₈,其余各组治疗作用明显,疗后与空白对照组比较均P>0.05。模型对照组血中免疫球蛋白IgA、IgG、补体C₃较空白对照组显著上升,P<0.01。IgM亦上升,P<0.05。除妇痛宁低剂量组,各组疗后数值均处于正常水平,与空白对照组比较P>0.05。IgE在各组间无明显变化。[结论]活血化瘀、软坚散结法的代表方妇痛宁,对子宫内膜异位症免疫功能异常具有显著调整作用,并可能通过此途径达到治疗目的。

Abstract:

[Objective] To explore the immunological mechanism of Futongning Granule in treating Endometriosis (EMS). [Methods] The rat model of EMS was made by surgical implantation. All rats were divided into 6 groups. T cell subpopulation in peripheral blood, immunoglobulin A (IgA), IgG, IgM, IgE and Complement 3 (C₃) in serum were detected. [Result] 1) CD₃, CD₄ and CD₈ in model group were different to that in control group, P<0.01. The effect of each group has no difference with that in control group except CD₄ in danazol group and CD₈ in Futongning low dosage treatment group, P>0.05. 2) IgA, IgG and C₃ in model group was higher than that in control group, P<0.01. IgM was also enhanced, P<0.05. The data in each treatment group except Futongning low dosage group were normal, compared with that in blank control group, P>0.05. There was no difference of IgE between each group. [Conclusion] Futongning Granule can regulate the immunological capacity in EMS.