中药扶正合剂介导白血病生物治疗的临床及实验研究

Clinical and experimental research of Chinese medicine Fuzheng Mixtureon biological and mediated treatment of leukemia

关键词:  中药扶正合剂  急性白血病  生物治疗  细胞免疫  L615白血病小鼠

Key words:  Fuzheng Mixture  acute leukemia  biological treatment  cellular immunity  L615 mice in leukemia

摘要:

[目的]探讨中药扶正合剂介导白血病生物治疗的可行性。[方法]观察30例急性白血病患者联合应用化疗及中药扶正合剂后,临床症状改善,造血功能和免疫功能重建的变化。复制L₆₁₅小白鼠白血病动物模型,从细胞免疫学角度探讨中药扶正合剂介导白血病生物治疗的作用机制。[结果]中药扶正合剂可通过促进IL-2、IL-6、IFN-γ等细胞因子的分泌水平,恢复急性白血病患者化疗后的免疫功能。可增强造血调节因子(GM-CSF)、IL-6的活性,加速骨髓再生,改善外周血象,减轻贫血、出血、感染等症状。延长L₆₁₅白血病小鼠的生存期,调整紊乱T细胞亚群,提高小鼠化疗后IL-2、IL-6、IFN-γ的分泌水平。[结论]中药扶正合剂可有效地介导白血病生物治疗。

Abstract:

[Objective] To explore the probability of the mediated and biological treatment of leukemia by Chinese medicine Fuzheng Mixture. [Methods] Thirty cases with acute leukemia were treated with combined chemotherapy and Fuzheng Mixture. The changes of clinical symptoms, hematopoiesis and restitution of immunologic function were observed. The model of leukemia in L₆₁₅ mice were reproduced. The mechanism of the mediated and biological treatment of leukemia by Fuzheng Mixture were studied from the aspect of cellular immunity. [Result] Chinese medicine Fuzheng Mixture could improve the immunologic function of the patients with acute leukemia after chemotherapy by promoting the secretion of cytokine such as IL-2, IL-6, IFN-γ, and so on. It could also improve the activity of hematopoietic regulatory factors (GM-CSF) and IL-6, accelerate bone marrow regeneration, improve the peripheral hemogram and eliminate anemia, hemorrhage and infection. In the experimental research, the survival time of L₆₁₅ leukemia mice were prolonged and the secretion of IL-2, IL-6 and IFN-γ was enhanced by regulating the disturbed T cell subpopulation. [Conclusion] Chinese medicine Fuzheng Mixture can effectively mediated the biological treatment of leukemia.