四物汤及两味中药组方对γ射线照射所致血虚证小鼠作用机制的研究

Mechanism research of Siwu Decoction and every combination of two herbs in it on mice with blood deficiency syndrome induced by γ-ray

关键词:  四物汤  血虚证  CD34⁺细胞  NFS60细胞

Key words:  Siwu Decoction  blood deficiency syndrom  CD34⁺ cell  NFS60 cell

摘要:

[目的]探讨四物汤及其两味药组方治疗血虚证小鼠的补血作用机制。[方法]采用⁶⁰Coγ射线全身照射制成实验性小鼠血虚证模型,测定外周血象、CD34⁺细胞、细胞周期、NFS60细胞增殖以及造血干祖细胞的增殖情况。[结果]1)四物汤在各指标上都优于其两味药组方;(2)两味药组方中,当归相关组对CFU-E,CFU-Mix,CFU-GM的促进作用较好,且有升高白细胞的作用;而地黄相关组对CFU-E有增殖作用;白芍相关组能明显促进NFS60细胞的增殖;川芎相关组增加CD₃4抗原表达,并促进细胞由G_0/1期向S期转化,其作用和所含的川芎嗪有关。[结论]四物汤全方优于两味药组方,各组方的作用靶点和作用强度各有异同。

Abstract:

[Objective] To study the mechanism of Siwu Decoction and every combination of two herbs in it on mice with blood deficiency syndrome. [Methods] The blood deficiency model was by systemic irradiation of ⁶⁰Coγ-ray on mice. Peripheral hemogram, CD34⁺ cell, cell cycle, proliferation of NFS60 cell and hemopioetic cell were observed. [Result] 1) All indexes of Siwu Decoction was superior to that of other combination. 2) The combination related with Danggui could improve CFU-E, CFU-Mix and CFU-GM and enhance WBC. The combination related with Dihuang could promote the proliferation of CFU-E. The combination related with Baishao could improve the proliferation of NF60 cell markedly. The combination related with Chuanxiong could promote CD34 antigen expression and accelerate cell transformation from G_0/1 phase to S phase. The effect of the last combination was associated with ligustrazine. [Conclusion] The whole decoction, Siwu Decoction is superior to other combination. The effect target and the effect strength of each combination is different.