| 摘要: |
| [目的]观察羊栖菜多糖对人胃癌细胞内Ca2+含量的变化,揭示其诱导肿瘤细胞凋亡的机制。[方法]采用Fluo-3/AM探针标记,激光共聚焦技术观测细胞内[Ca2+]i。[结果]SFPS可使SGC-7901细胞内[Ca2+]i先升高然后下降,给CaCl2后,[Ca2+]i又升高。[结论]SFPS诱导肿瘤细胞凋亡是通过升高SGC-7901细胞内[Ca2+]i而达到的,[Ca2+]i升高时Ca2+来源于细胞内钙库释放。 |
| 关键词: 羊栖菜多糖 SGC-7901 细胞内[Ca2+]i 作用机制 |
| DOI：10.11656/j.issn.1672-1519.2003.04.33 |
| 分类号: |
| 基金项目:国家自然科学基金资助课题(30271598);教育部科技研究重点项目(01035);黑龙江省普通高等学校骨干教师创新能力计划(2002015);黑龙江省自然科学基金(D00-18) |
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| Influence of SFPS on [Ca2+]i of SGC-7901 |
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GAO Shi-yong, JI Yu-bin
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Postdoctoral Programme, Institute of Material Medica of Harbin University of Commerce, Harbin 150076, China
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| Abstract: |
| [Objective] To study the change of Ca2+ in SGC-7901, for exploring the mechanism of apoptosis induced by SPFS. [Methods] Intracellulari [Ca2+]i was measured with laser confocal microscopy using Fluo-a/Amoribe labeling technique. [Result] SFPS, increased [Ca2+]i first and then declined. After giving CaCl2, i rise again. [Conclusion] SFPS can induce apoptosis of cancer cells through enhancing [Ca2+]i in SGC-7091. This kind of rises of Ca2+ is from the release of cellular storage. |
| Key words: SFPS SGC-7901 [Ca2+]i action of effect |
