活血承气合剂对小肠缺血-再灌注家兔血清二胺氧化酶的影响

Alteration of serum diamine oxidase and effect of HXCQ Decoction in rabbit intestinal ischemia-reperfusion

关键词:  小肠  缺血再灌注  二胺氧化酶  肠衰竭  中西医结合

Key words:  intestine  ischemia_reperfusion  diamine oxidase  intestinal failure  Integrated traditional and western medicine

摘要:

[目的]以血清二胺氧化酶(Diamine Oxidase,DAO)作为判断小肠损伤程度的指标,通过制作实验性家兔小肠缺血再灌注(ischemia-reperfusion,I/R)模型,评价血清DAO诊断小肠损伤的可靠性和有效性,并依此指导治疗,探讨应用中西医结合方法防治小肠缺血再灌注损伤的效果。[方法]采用肠系膜上动脉夹闭方法制作I/R模型,将家兔随机分为假手术组,生理盐水对照组,活血承气合剂中药组,乳果糖组,每组再分为缺血60min、再灌注60min、再灌注120min 3个时间点,每组6只动物,取血检测血清二胺氧化酶活性。[结果]小肠缺血后血清DAO升高,于再灌注120min最高(P<0.05);应用活血承气合剂和乳果糖后血清DAO值升高则不明显;应用活血承气合剂组血清DAO水平明显低于生理盐水对照组(P<0.05);再灌注120min时,活血承气合剂组血清DAO显著低于乳果糖组(P<0.05)。[结论]小肠缺血再灌注损伤后血清DAO水平明显升高;应用中药活血承气合剂治疗可以显著降低血清DAO水平,改善缺血再灌注小肠的功能。

Abstract:

[Objective] To evaluate serum diamine oxidase as a marker of intestinal injury and dysfunction by rabbit intestinal ischemia-reperfusion model. We also investigated the effect of HXCQ decoction on this model.[Methods] Rabbit model of intestinal I/R injury was established with clamping of the superior mesenteric artery for 60 min and then clamping was relieved for 60 min or 120 min.Animals were divided into 10 groups randomly, pseudo-operation group, three treatment groups(Ns, HXCQ decoction and lactulose). Three treatment groups included three subgroups;ischemia 60 min, reperfusion 60 min,reperfusion 120 min. Each group contained 6 animals. Serum was obtained for detection of diamine oxidase.[Results] There was low level of serum diamine oxidase in normal animal. Serum DAO was significantly elevated after intestinal I/R injury (P<0.05). But when administrated with HXCQ decoction, serum DAO was lower than with NS and lactulose(P<0.05).[Conclusion] Serum DAO is a good maker of intestinal injury. Traditional Chinese medicine HXCQ decoction can protect intestine from I/R injury.