| 本文已被：浏览 2203次 下载 2442次 |
码上扫一扫！ |
|
|
| 四物汤及其提取物对辐射致血虚证小鼠造血作用的研究 |
|
高月1, 马增春1, 谭洪玲1, 佟丽1, 戴维群2, 肖成荣1, 王升启1
|
|
1.军事医学科学院放射医学研究所, 北京, 100850;2.吉林大学中日联谊医院, 长春, 130023
|
|
| 摘要: |
| [目的]探讨四物汤及其提取物治疗辐射致血虚证小鼠的作用机制和有效物质基础。[方法]γ射线全身照射小鼠制成血虚证模型,观察四物汤对其外周血和造血祖细胞集落的影响,基因芯片检测有效单体芍药苷的分子作用机制。[结果]四物汤、正丁醇提取物、水溶提取物对血虚证小鼠外周的白细胞的恢复有明显促进作用;四物汤和芍药苷增强血虚证小鼠骨髓造血干祖细胞的集落形成能力;芍药苷可促进骨髓基质细胞分泌造血因子(G-CSF,GM-CSF,PDGF-α)等,抑制造血抑制因子(M-CIP)的分泌。[结论]四物汤补血的物质基础可能是芍药苷,促进骨髓基质细胞分泌G-CSF,GM-CSF,PDGF-α等造血因子可能是四物汤补血的作用机制之一。 |
| 关键词: 四物汤 血虚 造血 基因芯片 |
| DOI：10.11656/j.issn.1672-1519.2003.06.23 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(30071913);北京市科技项目(H010210220113) |
|
| Effects of Si-Wu-Tang and its extracts on medullary hematopoiesis in mice of blood deficency induced by 60Coγ irradiation |
|
GAO Yue,MA Zeng-chun,TAN Hong-ling
|
|
Institute of Radiation Medicine, Beijing 100850, China
|
| Abstract: |
| [Objective] To study the mechanism and active constituents of Si-Wu-Tang on the blood deficiency induced by using 60Coγ irradiation.[Methods] Blood deficient mice were established by using 60Coγ irradiation, the ability of colony-forming unit of bone marrow cells and the number of circulating blood cells have been observed, the molecule mechanism of Paeoniae on enriching blood was investigated by gene chip.[Results] Si-Wu-Tang and its butyalcohol and h2o extracts could increase the numbers of circulating white blood cells, Si-Wu-Tang and Paeoniae could increased the ability of colony-forming, GM-CSF, G-CSF, PDGF-α increased and MIP decreased in stromal cells treated by Paeoniae.[Conclusion] Paeoniae might be one of active constituents of Si-Wu-Tang on the blood deficiency, stimulated producing GM-CSF, G-CSF,PDGF-α in stromal cells mignt be the mechanism of SWT on the BD mice. |
| Key words: Si_Wu_Tang blood deficiency hematopoiesis gene chip |
