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| 合成冰片对沙门氏菌的诱变作用观察 |
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刘洋1, 李怡岚2, 胡利民1, 范祥1, 周蕾2, 张伯礼1
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1.天津中医学院, 天津, 300193;2.天津市卫生防病中心, 天津, 300100
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| 摘要: |
| [目的]观察合成冰片是否有诱发沙门氏菌株发生基因突变的作用。[方法]采用鼠伤寒沙门氏菌营养缺陷型回复突变试验(Ames试验)。[结果]合成冰片在0.4~250μg/皿范围内,无论有或无肝脏微粒体酶(S9)活化系统,对菌落回变无明显影响,且回变菌落背景正常。[结论]合成冰片对鼠伤寒沙门氏菌突变株无致突变作用,具有临床应用的安全性。 |
| 关键词: 合成冰片 诱变性(Ames)试验 致突变性 |
| DOI：10.11656/j.issn.1672-1519.2003.06.30 |
| 分类号: |
| 基金项目:国家重点基础研究课题项目 (973)——方剂关键科学问题的基础研究(G1999054403);天津市科委重大科技攻关项目《一种新来源天然冰片的特殊毒性研究》资助 (编号013613711) |
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| Study for mutagenic of Synthetic borneol |
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LIU Yang,LI Yi-lan,HU Li-min
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Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To observe the mutagenicity effect of Synthetic Borneol.[Methods] Ames test was used.[Results] The number of backward mutant colony of four bacterial strains induced by Synthetic Borneol at the concentration within 0.4~250 μg/plate was quantified. The results did not display a significant increase compared with that induced by dimethylsulfoxide, whether with metabolic activation system (S9-mix) or not. Moreover, the backward mutant colony had normal background.[Conclusion] Synthetic Borneol did not possess evident mutagenicity through our study. |
| Key words: synthetic borneol ames test mutagenicity |
