大承气汤和大黄对巨噬细胞免疫活性的双向调节作用

Bidirectional regulation of Dachengqi Decoction and Rhubarb on immunologic activity of macrophages

关键词:  大承气汤  大黄  细菌脂多糖  巨噬细胞  肿瘤坏死因子α

Key words:  Dachengqi Decoction  rhubarb  lipopolysaccharide  macrophages  tumor necrosis factor α

摘要:

[目的]研究大承气汤(DCQ)及其主药大黄(DH)对正常的和脂多糖(LPS)刺激的大鼠腹腔巨噬细胞(PMΦ)释放肿瘤坏死因子α(TNFα)作用的特征。[方法]利用对TNF敏感的细胞系L929,用MTT法检测PMΦ释放的TNFα量。[结果]LPS刺激PMΦ释放TNFα在8h达到峰值,其峰值随LPS浓度增加而增加;低浓度DCQ和DH对正常PMΦ释放TNFα有轻度激活作用;DCQ和DH对LPS刺激PMΦ过量释放TNF(有显著抑制作用,其效应随药物浓度的增加而增强,并且抑制作用主要表现在给药后的早期阶段。[结论]DCQ及其主药DH对PMΦ释放TNFα有双向调节作用。

Abstract:

[Objective] To study the effect of Dachengqi Decoction (DCQ) and Rhubarb on the release of tumor necrosis factor α (TNFα) from normal or LPS-stimulated rat peritoneal macrophages (PMΦ). [Methods] TNFαwas assayed by MTT method, using the TNFα-sensitive cell line L929. [Result] The release of TNFαfrom PMΦstimulated by LPS could reach a peak after 8 hours, the peak was increasing with the LPS concentration increasing. Low concentrations of DCQ and Rhubarb could activate normal PMΦto release TNFαslightly. However, DCQ and Rhubarb evidently inhibited the excessive release of TNFαfrom PMΦstimulated by LPS, and the inhibition enhanced with the increasing of drug concentration. The inhibition mostly appeared in early phase after drug administration. [Conclusion] DCQ and Rhubarb have bidirectional regulation on the release of TNFαfrom macrophages.