| 摘要: |
| [目的]采用高效液相色谱法测定养阴清肺颗粒中丹皮酚的含量。[方法]采用C18(4.6mm×250mm,10μm)色谱柱,甲醇-水(75:25)为流动相,流速1.00mL/min,检测波长274nm,外标法定量。[结果]丹皮酚在0.0413～0.2065μg范围呈线性,回收率为98.8%,峰面积(RSD)为1.02%(n=6)。[结论]液相色谱法简便可行,专一性强,能够有效控制该制剂的质量。 |
| 关键词: 养阴清肺颗粒 丹皮酚 高效液相色谱 |
| DOI：10.11656/j.issn.1672-1519.2004.01.37 |
| 分类号: |
| 基金项目: |
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| Determination of Paeonol in Yangyinqingfei Granula by HPLC |
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LI Jian, CHEN Yan, XU Jin-bo
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Tianjin Tasly Pharmaceutical Co.Ltd, Tianjin 300142, China
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| Abstract: |
| [Objective] To develop a method using HPLC for determination content of Paeonol. C18 column (4.6 mm×250 mm,10 μm) was used. [Methods] Methanol-water (75:25) was used as mobile phase. The flow rate was 1.00 mL/min. Detection was carried out at 274nm.The external standard method was used. [Results] It has a good linear correlation in 0.0413～0.2065 μg (r=0.9998),the average recovery was 99.8% (RSD=1.02%,n=6). [Conclusion] This method provided simple and rapid method to analyze Paeonol in Yangyinqingfei Granula. |
| Key words: Yangyinqingfei Granula Paeonol HPLC |
