冰片对大鼠血浆儿茶酚胺类物质影响的实验研究

Effect of borneol on plasma catecholamines in rats

关键词:  冰片  高效液相-电化学法  儿茶酚胺  去甲肾上腺素  肾上腺素

Key words:  borneol  high performance liquid chromatograpy(HPLC)electrochemical assay  catecholamine(CA)  norepinephrine(NE)  adnephrin

摘要:

[目的]观察冰片对大鼠血浆儿茶酚胺(CA)类物质去甲肾上腺素(NE)、肾上腺素(E)含量的影响,同时进行量效关系的观察,初步探讨冰片的作用靶点和作用机制。[方法]将40只wistar大鼠随机分组后,分别灌服生理盐水,1%羟甲基纤维素钠(CMC-Na),以及高、中、低剂量的冰片,2次/d,灌胃24h后,用高效液相-电化学法检测大鼠血浆儿茶酚胺类物质NE、E含量。[结果]1)灌服冰片24h后,冰片各剂量组血浆NE含量均有下降,其中冰片中组、高组和CMC-Na组相比有统计学差异(P<0.05);2)灌服冰片24h后,冰片各组与CMC-Na组相比呈现降低作用,其中冰片中剂量组有统计学性差异(P<0.05)。[结论]冰片对于交感神经的兴奋性和儿茶酚胺类物质释放有一定抑制作用,据此推测冰片可能通过影响神经内分泌功能,进而调节机体的生理病理活动,这可能是临床上广泛应用含冰片的制剂防治心血管疾病的机制之一。

Abstract:

[Objective] To investigate the effect of borneol on the plasma contents of catecholamines NE,E in rats and observe the relationship of dosage-effect to explore the mechanisms and target of borneol.[Methods] 40 wistar rats were randomly divided into 5 groups,0.9%NS,1%CMC-Na,and natural borneol in different dosages were given respectively twice a day. 24 hours later,the contents of catecholamines NE,E levels of plasma in rats were measured by HPLC combined with electrochemical assay.[Results] 1)Decreased NE contents in plasma were observed in each borneol group with significant difference between middle-dosage,high dosage groups and CMC-Na group(P<0.05);2)Compared with CMC-Na group,decreased levels were seen in every borneol group with marked difference in middle-dosage group(P<0.05).[Conclusion] borneol has certain inhibitory action on excitabilitv of sympathetic nerve and release of catecholamines. It is estimated that borneol can regulate the physiopathologic activity of organism probably through affecting neuroendocrine function. This may be one of the mechanisms for using borneol preparations extensively in clinic to prevent and treat cardiovascular disease.