壮骨止痛胶囊含药血清对大鼠成骨样细胞增殖分化影响的实验研究

Effect of Zhuanggu Zhitong Capsule on the proliferation and differentiation in rat of osteoblast

关键词:  成骨细胞  壮骨止痛胶囊  含药血清  增殖  分化

Key words:  osteoblast  Zhuanggu Zhitong capsule(ZZC)  Serum with absorbed Zhuanggu Zhitong capsule  proliferation  differentiation.

摘要:

[目的]观察壮骨止痛胶囊含药血清对成骨细胞增殖、分化的影响,为明确壮骨止痛胶囊防治骨质疏松症的作用机制提供实验依据。[方法]取2代培养的大鼠成骨细胞,在壮骨止痛胶囊含药血清为40%的浓度中培养。观察细胞的生长及钙结节的形成,碱性磷酸酶(ALP)测定成骨细胞增殖和分化,噻唑蓝(MTT)法测成骨细胞生长曲线。[结果]成骨细胞在6d可铺满瓶壁,30d可形成钙结节,壮骨止痛胶囊含药血清可促进成骨细胞的增殖、ALP的分泌。[结论]壮骨止痛胶囊含药血清可促进大鼠成骨细胞的增殖、分化。壮骨止痛胶囊含药血清可能通过直接促进成骨细胞增殖、分化,从而防治骨质疏松症。

Abstract:

[Objective]To observe the effect of Zhuanggu Zhitong capsule(ZZC)on the proliferation and differentiation in rat of osteoblast,and provide experimental evidence on the mechanism for treatment of osteoporosis.[Methods]The second generation of rat osteoblast was cultured in medium with 40% ZZC-contained serum,and cell growth and the formation of calcium nodule were observed.Growth curve of osteoblast was detected by MTT method,while differentiation and prolif-eration were determined by measuring alkalinephosphtase(ALP)activity.[Result]Serum with absorbed ZZC obviously in-creased osteoblast proliferation and differentiation and the secretion of ALP.[Conclusion]Serum with absorbed ZZC can promote the proliferation and differentiation of osteoblast in rats.It is probably the mechanism in preventing and curing the osteoporosis.