强骨丹对去卵巢大鼠骨质疏松症防治作用的实验研究

Preventive and Therapeutic Effect of Qianggu Dan on Osteoporosis in Ovariectomized Rats

关键词:  强骨丹  绝经后骨质疏松症  实验研究

Key words:  Qinggu Dan  postmenopausal osteoporosis  bone metabolism

摘要:

[目的]探讨中药强骨丹对绝经后骨质疏松症的防治作用。[方法]运用切除大鼠卵巢方法建立去卵巢骨质疏松症模型,随机分为模型组、强骨丹高低剂量组、西药组,同时设假手术对照组。采用双能X线骨密度测定法、放射免疫分析法和ELISA法等检测各组实验鼠骨密度 (BMD)、骨矿含量 (BMC)、血清雌二醇 (E₂)、骨钙素 (BGP)、抗酒石酸酸性磷酸酶5b(TRACP5b)以及尿脱氧吡啶啉 (Dpol)。[结果]强骨丹可明显升高BMD、BMC,提高血清E₂ 水平,降低TRACP5b和Dpd水平,并降低BGP含量。[结论]强骨丹通过调节机体相关内分泌功能,抑制骨吸收,降低骨的转换率,减少骨丢失,增加骨密度,对绝经后骨质疏松症有防治作用。

Abstract:

[Objective] To investigate the preventive and therapeutic effect of Qianggu Dan(Strengthening bone pill)on the postmenopausal osteoporosis. [Methods] The disease was induced by ovariectomy.in rats.They were randomly divided into5groups:model group,Qianggu Dan high dosage group and low dosage group,western medicine group and the sham op-eration group.Bone mineral density(BMD),bone mineral content(BMC),serum estradiol(E₂),bone calcium plain(BGP),tartaric resting acid phosphorus5b(TRACP5b) and urine deoxypyridinoline(Dpd)in each group were detected by double energy X-ray(DEXA),RIA and ELISA. [Results] Qianggu Dan can obviously increase the level of BMD,BMC and serum E₂ and reduce TRACP5b and Dpd in serum.It can also lower the content of BGP. [Conclusion] Qianggu Dan may inhibit bone absorption,lower the rate of the bone transformation,and reduce the bone losing by regulating the function of cor-relative endocrine glands,so has a significant preventive and curative effect on postmenopausal osteoporosis.