| 摘要: |
| [目的]建立测定痔可消胶囊中大黄酸含量的方法。[方法]采用ODS柱以甲醇-水-醋酸 (83:17:0.2)为流动相,用高效液相色谱法测定,检测波长254nm。[结果]大黄酸进样量在0.1～0.5μg 线性良好,r=0.9998,平均回收率为99.97%,相对标准偏差 (RSD)为1.91%。[结论]此方法简便、准确、重现性好,可作为制剂及药材的定量方法。 |
| 关键词: 痔可消胶囊 大黄酸 含量测定 高效液相法 |
| DOI：10.11656/j.issn.1672-1519.2004.05.30 |
| 分类号: |
| 基金项目: |
|
| Content determination of Rhein in Zhikexiao Capsule by HPLC |
|
GAO Jing,CHEN Zhi-juan,ZHANG Shu-hua
|
|
Tianjin Academy of Pharmaceutical Research, Tianjin 300193, China
|
| Abstract: |
| [Objective] To establish a method for the content determination of Rhein in Zhikexiao Capsule. [Methods] A simple HPLC method for determination of Rhein was established.The determination was carried out on ODS column with mobile phase of methanol-water-acetic acid(83:17:0.2)and detection wave-length at 254nm. [Result] The Rhein sample size showed a good linear relationship at the range of 0.1～0.5μg and correlation coefficient was 0.9998.The average re-covery rate of the added sample was 99.97%.RSD was 1.91%. [Conclusion] The method is simple,accurate,reproducibe and can be used for content determination of preparation and medicinal materials. |
| Key words: Zhekexiao Capsule Rhein content determination HPLC |
