丹酚酸B预适应对心脏微血管内皮细胞保护作用的研究

Protective Effect of Preconditioning with Salvianolic Acid B Against Cardiac Microvascular Endothelial Cell Injury

关键词:  预适应  缺O₂/复O₂  细胞保护  丹酚酸B

Key words:  preconditioning  hypoxia-reoxygenation  protective effect against cell injury  salvianolic acid B

摘要:

[目的]研究丹酚酸B预适应的心脏细胞保护作用及机制。[方法]基于缺氧预适应(HPC)机制,通过心脏微血管内皮细胞(CMEC)体外培养技术,建立缺O₂/复O₂损伤的细胞模型,以细胞活力、乳酸脱氢酶(LDH)、一氧化氮(NO)、超氧化物歧化酶(SOD)、内皮素(ET)等生化指标作为评价标准。[结果]在正常培养条件下,丹酚酸B可明显提高CMEC和心肌细胞的细胞存活率和SOD活性、降低LDH活性,有降低CMEC的ET分泌和NO释放趋势。缺O₂/复O₂损伤情况下,丹酚酸B不仅能提高CMEC存活率和SOD活性,降低LDH活性,而且能明显降低其因缺O₂/复O₂导致的ET和NO水平升高。缺O₂预适应可增强CMEC对随后较长时间缺O₂/复O₂损伤的耐受性,表现为可提高CMEC存活率和SOD活性,降低LDH活性,降低ET和NO分泌。[结论]丹酚酸B预适应具有缺O₂预适应样作用,从而激发了CMEC的内源性保护机制。

Abstract:

[Objective] To study the protective effect and mechanism of preconditioning with salvianolic acid B on the cardiac microvascular endothelial cells (Cmec).[Methods] We established a damage model of hypoxia-reoxygenation on CMEC in vitro by cell cultrue technique The protective effect on the cell exposed to salvianolic acid B, preconditioning,was examined with biochemistry.The rate of live cell (MTT),lactate dehydrogenase (LDH),superoxide dismutase (SOD),nitric oxide (NO),endothelin (ET) etc.were used as the evaluating parameters.[Results] Salvianolic acid B could markedly improve the survival rate of CMEC and cardiac cell,rise the activity of SOD,decrease the activity of LDH,had a tendency to lower the secretion of ET and release of NO.After hypoxia-reoxygenation salvianolic acid B could not only increase CMEC survival and SOD activity decrease LDH activity but also markedly decrease the increasing level of ET and NO resuled from hypoxia-reoxygenation.[Conclusion] Savianolic acid B conditioning has a similar effect as hypoxia-reoxygenation.