| 摘要: |
| [目的]从调节白细胞内皮细胞过度黏附角度研究活血化瘀中药的作用机制。[方法]体外培养肺微血管内皮细胞,RT-PCR方法观察活血化瘀注射液对肿瘤坏死因子-α(TNF-α)诱发的体外培养大鼠肺微血管内皮细胞E-选择素、P-选择素、细胞间黏附分子ICAM-1mRNA表达的影响。[结果]肺微血管内皮细胞与含TNF-α培养基共同孵育后,可显著增加内皮细胞E-选择素、P-选择素、ICAM-1mRNA表达,在培养基中加入活血化瘀注射液含药血清后,则内皮细胞E-选择素、P-选择素、ICAM-1mRNA过度表达被明显抑制。[结论]抑制内皮细胞E-选择素、P-选择素、ICAM-1mRNA过度表达是活血化瘀重要的作用机制之一。 |
| 关键词: 活血化瘀注射液 肺微血管内皮细胞 细胞因子 黏附分子 |
| DOI：10.11656/j.issn.1672-1519.2005.03.32 |
| 分类号: |
| 基金项目:天津市卫生局科研基金资助课题(98ky-9) |
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| Effect of HHI-I on TNF-alpha Mediated Expression of Adhesion Molecules mRNA of Pulmonary Microvascular Endothelium in Rats |
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ZHANG Yan-Jun,ZHAO Lian-gen,WU Xiang-zhong
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Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To elucidate the mechanism underlying the effect of Huoxue Huayu(Activating blood circulation and remove stasis) herbal medicine on the recruitment of leukocytes in blood circulation. [Methods] The effect of HHI-I(Huoxue Huayu Injection No.I) on the expression of adhesion molecules mRNA induced by TNF-alpha was investigated in vitro with RT-PCR technique. [Results] In the cultured lung microvascular endothelial cells stimulated with TNF-alpha, the expression of adhesion molecules, such as E-selectin, P-selectin, ICAM-1 mRNA, was increased significantly. In contrast, the cells exposed to HHI-I simultan eously exhibited a marked lower expression of the TNF-alpha induced endothelial adhesion molecules. [Conclusion] HHI-I can inhibit the excessive expression of endothelial cell adhesion molecules. |
| Key words: lung microvascular endothelial cell cytokine adhesion molecule HHI-I |
