腺嘌呤致不同性别大鼠骨质疏松的差异

Sex Differencien Osteopenia Induced by Adeninein Rats

关键词:  腺嘌呤  骨质流失  性别差别  睾酮  17-βHSD

Key words:  adenine  bone loss  sex difference  testosterone  17beta-HSD

摘要:

肌酸尿时多见而又为主要病因的腺嘌呤广泛地应用于临床,但是腺嘌呤过多对人体是有害的。到目前为止,只有应用雄性动物研究腺嘌呤影响的报告。在雄鼠发现喂饲富含腺嘌呤的饲料会引起肾衰竭。由于腺嘌呤对雌性动物的影响的报告还甚少,本实验研究了腺嘌呤处理对不同性别大鼠的影响,并进行了讨论。年轻雄性和雌性大鼠分别给与一种预定浓度的腺嘌呤(6、60及100mg/mL)共8周(3次/周)。结果100mg/mL腺嘌呤处理能引起雄鼠肾衰,50mg/mL能引起肾功能失调。但是,只有100mg/mL才能引起雌鼠肾功能失调,其影响与雄性大鼠有些不同。雄鼠血清睾酮水平及BMD可因腺嘌呤处理而降低,但肾功能失调可有可无。相反,雌鼠尽管有肾功能失调,血清17-β雌二醇水平及BMD可完全不受腺嘌呤处理的影响。如上所述,本研究能说明腺嘌呤对骨代谢的影响是通过性激素合成实现的。腺嘌呤常含于临床药物及日常食物中,过多摄入包括腺嘌呤在内的核酸会影响内分泌功能。

Abstract:

Adenine, which is common and essential to creatures, is widely used in clinical field. However, abundance of adenine is harmful to the living body. So far the male animals have been used for the research on adenine influence. In male rats, feeding of adenine-rich dietinduces renal failure. Because of very few studies of adenine influence to female animals,we investigated the influence of adenine treatment between male and female rats and discussed the sex difference on adenine treatment.Young male and female rats were administrated each adjusted adenine (6,50and 100mg/mL)for 8weeks (3times/week).In male rats,the renal failure was induced by 100mg/mL adenine treatment and renal dysfunction was induced by 50mg/mL adenine treatment.In female rats,however renal dysfunction was induced by 100mg/mL group only, and that was som ewhat different com pared to that in male rats. Serum testosterone level and BMD in male rats were decreased by adenine treatment with or without renal dysfunction. On the contrary, serum 17-beta estradiol level and BMD in female rats were not affected by adenine treatment at all regardless of renal dysfunction.As abovem entioned,this study could shed light on adenine effect on bone metabolism through sex hormone synthesis. A denine is commonly contained in clinical medicines and general food.Abundant ingestion of nucleic acid including adenine may affect the internal secretion function.