活血化瘀注射液I号对小鼠致死性缺氧的保护作用

Protective Effect of Huoxuehuayu Injection I Against Lethal Hypoxic Injury in Mice

关键词:  缺氧  脑水肿  肺水肿  存活时间  小鼠  活血化瘀注射液Ⅰ号(HHI-Ⅰ)

Key words:  hypoxia  brain edema  lung edema  survival time  mice

摘要:

[目的]观察已知有促进脑血液循环作用的活血化瘀注射液I号(HHI-I)对小鼠致死性缺氧(5%)的保护作用。[方法]昆明种小鼠40只,分为4组,每组10只,雌雄各半。实验日给动物分别腹腔注射生理盐水(对照组)、1.5%、3%、6%HHI-I,0.5mL/只。1h后将动物置5%缺氧小室内,观察1h内存活时间,1h后分别取标本测定脑及肺组织水含量。[结果]与对照组比较,6%HHI-I可明显降低动物脑组织水含量,有降低肺组织水含量的趋势,对存活时间无明显影响。3%及1.5%HHI-I无类似作用。[结论]6%HHI-I对小鼠致死性缺氧条件下脑水肿形成有明显预防作用。

Abstract:

[Objective] To observe the protective effect of Huoxuehuayu injection I HHI.I against lethal hypoxic injury in mice. [Methods] Forty Kunming mice were randomly divided into four groups.Ten mice were in each group with male and female distributed equally.During the experiment,0.9% saline control group,1.5% HHI.I low dosage group,3.0% HHI.I moderate dosage group and 6% HHI.I high dosage group,0.5 mL per mouse,were administrated respectively.One hour latter,the mice were put into a 5% hypoxic chamber and recorded their survival time in one hour period.After that,the water content of the brain and lung tissue samples was determined. [Result] Compared with the control group,6% HHI.I group could markedly decrease the water content in brain P<0.01.A tendency of decreasing of water content in lung was observed as well.But the difference in survival time wasn’t significant between groups.However,3% and 1.5% HHI.I group didn’t have the similar actions. [Conclusion] The 6% HHI.I has significant preventive effect against the brain edema formation induced with 5% hypoxia in mice.