芪玄益心胶囊对糖尿病大鼠缺血心室肌细胞动作电位及瞬时外向钾电流的影响

Effect of Qixuanyixin Capsule on Action Potential and Transient outward potassium channel of Ischemic Ventricular Muscle Cell in Diabetes Rats

关键词:  芪玄益心胶囊  糖尿病  动物模型  膜片钳  动作电位  瞬时外向钾电流  大鼠

Key words:  Qixuanyixin Capsule  diabetes  animal model  patch clamp  action potential  transient outward potassium channel  rats

摘要:

[目的]探讨芪玄益心胶囊对糖尿病(DM)大鼠急性缺血心肌瞬时外向钾电流的影响,从细胞通道水平探讨该药的治疗机制。[方法]以链脲佐菌素(STZ)腹腔注射致胰腺损伤合并舌下静脉注射垂体后叶素使冠状动脉收缩,建立DM急性心肌缺血动物模型,采用全细胞膜片钳技术,记录芪玄益心胶囊对单个大鼠心室肌细胞动作电位(AP)相关指标及对瞬时外向钾电流1(I_to1)的影响。[结果]芪玄益心胶囊高剂量组心室肌细胞动作电位相关指标、瞬时外向钾电流密度较模型组明显改善,同模型组比较具有显著意义(P<0.05)。[结论]芪玄益心胶囊能够加大I_to1的开放,并推测芪玄益心胶囊抗心肌缺血的作用与开放瞬时外向钾电流,改善动作电位,进而产生类似缺血预处理的心肌保护作用密切相关。

Abstract:

[Objective] To study the effect of Qixuanyixin(supplementing heart)capsule on the action potential and transient outward potassium channel of ischemic ventricular muscle cell in diabetes rats,and investigate the therapeutic mechanism of Qixuanyixin capsule based on cell channel level.[Methods] The acute myocardial ischemia of diabetes rats was induced by intraperitoneal injection of streptozotocin(STZ)to cause pancreatic damage and pituitrin into sublingual vein to cause the contraction of coronary artery.The effect of Qixuanyixin Capsule on the action potential and transient outward potassium channel of ischemic ventricular muscle cell were recorded by the technique of whole cellular membrane patch clamp.[Results] The high dosage group of Qixuanyixin capsule could obviously improve the change of action potential index and transient outward potassium channel(Itol)with a significant difference when compared with model group(P<0.05).[Conclusions] Qixuanyixin Capsule can activate I_to1.It is considered that the anti-ischemia effect of Qixuanyixin capsule was associated with activitation of I_to1,and improvement of action potential and transient outward potassium channel.