针刺治疗脑出血时效关系的实验研究

Experimental Study on Time-effect Relationship in Treating Cerebral Hemorrhage with Acupuncture in Rats

关键词:  脑出血  针刺  时效性  脑细胞内Ca²⁺浓度  超氧化物歧化酶  丙二醛  兴奋性氨基酸

Key words:  cerebral hemorrhage  acupuncture treatment  Ca²⁺  SOD  MDA  EAA

摘要:

[目的]探讨针刺治疗脑出血大鼠的时效关系及其主要机制。[方法]采用二次二步注血法复制大鼠脑出血模型,观察不同时间介入的针刺治疗对脑出血模型大鼠的脑细胞内Ca²⁺浓度、脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)含量以及海马组织中兴奋性氨基酸(EAA)含量的影响。[结果]各针刺组的各项指标与模型对照组的差异具有显著性(P<0.05)。各针刺组之间比较表明,3h针刺组与9h针刺组各项指标的差异无显著意义(P>0.05),但与24h、48h针刺组各项指标之间的差异具有显著性(P<0.05);24h针刺组与48h针刺组各项指标的差异具有显著性(P<0.05)。[结论]针刺治疗对脑出血大鼠脑细胞具有良好的保护作用,而且早期针刺治疗的保护作用更强,提示临床治疗脑出血越早针刺效果越好。其主要机制包括:针刺治疗可以降低细胞内Ca²⁺的浓度,提高SOD的活力,减少MDA的生成,并且抑制海马组织中EAA的神经毒作用(P<0.05)。

Abstract:

[Objective] To explore the time-effect relationship and the main mechanism of acupuncture in treating cerebral hemorrhage in rats.[Methods] A model of cerebral hemorrhages in rats was established by using two steps of injection of autologous blood to observe the changes of intracellular free calcium(Ca²⁺),contents of SOD,MDA in brain tissue and the content of EAA in hippocampus tissue in different time after acupuncture respectively.[Results] We found that acupuncture could decrease the concentration of(Ca²⁺)in injured neural cells,increase the activity of SOD,reduce the production of MDA in brain tissue and inhibit the increasing of the content of EAA in hippocampus tissue.There was a statistic difference(P<0.05).There was a significant difference between the group of 3 hours treatment and the other three groups;and there was no significant difference between the group of 12 hours treatment,24 hours treatment and 48 hours treatment.[Conclusions] The acupuncture treatment possesses some protective effect against cerebral hemorrhage in rats.It can protect brain cells from cerebral hemorrhage in rats and the earlier treatment the better effect.