脑疏宁对大鼠脑出血性损伤的保护作用及机制研究

Protective Effects and Mechanism Research of Naoshuning on Brain Hemorrhage Injury in Rats

关键词:  脑出血  脑疏宁  神经功能缺损  脑水肿

Key words:  brain hemorrhage  Naoshuning  impairment of nervous function  brain edema

摘要:

[目的]观察脑疏宁对脑出血急性期大鼠的神经功能缺损、脑水肿的改善作用及其作用机制。[方法]将84只大鼠随机分为假手术组、脑出血组、脑疏宁低剂量组、脑疏宁中剂量组、脑疏宁高剂量组及甘露醇组,采用自体血回输方法建立大鼠脑出血模型,进行脑出血24h和48h后大鼠神经功能评分、木条行走作业能力和脑组织含水量测定。[结果]脑疏宁中剂量和高剂量对脑出血后神经功能缺损有明显改善作用;脑出血后48h脑疏宁中剂量和高剂量组的木条行走时间明显短于模型组且显著优于低剂量组;脑疏宁各治疗组与甘露醇组脑含水量明显低于模型组,与低剂量组相比,脑疏宁高剂量和甘露醇组的脑组织含水量明显降低。[结论]脑疏宁对大鼠脑出血后神经功能恢复和脑水含量均具有明显的改善作用,其作用机制可能是减轻血管源性脑水肿。

Abstract:

[Objective] To observe the improvement effects and the effect 8mechanism of Naoshuning prescription against the impairment of nervous function and brain edema in rats with acute brain hemorrhage rats.[Methods] Eighty-four rats were randomly divided into false operation group,brain hemorrhage group,lower dosage of Naoshuning group,middle dosage of Naoshuning group,high dosage of Naoshuning group,mannitol group.Brain hemorrhage model in rats was established by using autoblood transfusion method.The nervous function score,the walking ability on wood strips and the water content of brain tissue on the twenty-fourth and forty-eighth hour after brain hemorrhage were determined.[Results] The middle and high dosage group of Naoshuning may obviously improve nervous function impairment and the strip walking ability.The water content of Naoshuning and mannitol group was obviously lower than that of model group.[Conclusions] Naoshuning may obviously improve the impairment nervous function and decrease the brain water content.The effect mechanism may be related to lessening vasogenic brain edema.