清毒栓血清药理对宫颈癌SiHa细胞P53基因调控作用的研究

Serum pharmacology of Qingdu suppositorium and its regulating effect on SiHa cell P53 gene expression in cervix cancer

关键词:  清毒栓含药血清  宫颈癌SiHa细胞  P53

Key words:  Qingdu Suppositium serum  Cervical cancer SiHa cell  P53

摘要:

[目的] 研究中药清毒栓对宫颈癌SiHa细胞生长的调节作用,从细胞生物学及分子生物学水平探讨清毒栓抑制宫颈癌SiHa细胞生长的作用机制。[方法] 以不同浓度含药血清培养液作用于SiHa细胞,并以空白血清及含中、西药阳性对照药血清为对照,通过噻唑蓝(MTT)法检测该药对细胞生长及增殖的影响;用流式细胞分析技术进行细胞周期及细胞凋亡检测并用逆转录-聚合酶链反应(RT-PCR)法半定量检测对抑癌基因P53表达的影响。[结果] 经含药血清培养液作用后细胞数量减少,其中以8%浓度含药血清作用72h时对细胞抑制作用最强;细胞周期各时相中,S期细胞所占的比例明显减少;对细胞凋亡有影响但未见明显规律性,RT-PCR结果示细胞中P53表达明显增加。[结论] 清毒栓含药血清不仅可直接抑制宫颈癌细胞的生长,并可能通过调控P53基因表达的分子机制,达到抑制肿瘤的目的。

Abstract:

[Objective] To investigate the regulating effect of Qingdu Suppositium on the growth of SiH a cell in cervical carcinoma and explore the mechanism with cytobiology and molecular biology.[Methods] Medicine-contained serum with different concentration was cultured with SiHa cell,and the blank serum and the positive control containing Chinese or Western medicine were established also.The influence on cell growth and the proliferation was determined with MTT.The cell cycle and apoptosis were examined with flow cytometry and the influence on the expression of cancer-inhibiting gene P53 was examined with PT-PCR semi-quantitative method.[Results] The cell quantity was reduced after exposure to the medicine-contained serum.Te serum with the concentration of 8% and the exposure time of 72 h had the most intensive effect.The number of cell in S phase was reduced obviously.The Qingdu Suppositium serum could accelerate SiHa cell apoptosis withou significant regularity; P53 gene expression of cells was significantly upregulated.[Conclusions] The serum containing Qingdu Suppositium can not only suppress directly the growth of cervical cancer cell but also inhibit tumor by regulating the P53 gene expression.