穴位埋线对大鼠癫痫持续状态后海马神经元凋亡及相关基因表达的影响

Effect of acupoint catgut embedding therapy on hippocampal neurons apoptosis and apoptosis-associated genes expression in rats with status epilepticus

关键词:  癫痫  穴位埋线  海马神经元凋亡  基因表达

Key words:  Epilepsy  acupoint catgut embedding therapy  apoptosis  gene expression

摘要:

[目的]观察穴位埋线对大鼠癫痫持续状态(SE)后海马神经元凋亡及凋亡相关基因的影响。[方法]将40只SD大鼠随机分成正常组、造模组、埋线组、针刺组、西药组。用贝美格注射液腹腔注射法诱导大鼠癫痫持续状态。造模成功后48h,采用Tunel法和免疫组化法检测各组神经元凋亡及凋亡相关基因P53和B淋巴细胞-2(Bcl-2)的表达。[结果]与模型组相比,埋线组、针刺组、西药组的细胞凋亡指数及P53降低、Bcl-2增高。[结论]穴位埋线可能通过抑制P53蛋白,提高Bcl-2蛋白的表达,阻止海马神经元细胞凋亡的发生发展,减轻SE发生后对大脑的损害。

Abstract:

[Objective] To observe the effects of acupoint catgut embedding therapy hippocampal neurons apoptosis and the expression of associated genes P53 and Bcl-2 in rats with status epilepticus (SE). [Methods] Forty SD rats were randomized into normal group, model group, catgut embedding group, acupuncture group and positive control group. Rat models with SE were established by intraperitoneal injection of bemegride. Forty-eight hours after the establishment of the models, hippocampal neurons apoptosis and the expression of associated genes P53 and Bcl-2 in the four groups were observed by Tunel method and immunohistochemical method. [Results] Compared with the model group, the apoptotic index and P53 protein expression were decreased, and Bcl-2 protein expression increased in catgut embedding group, acupuncture group and positive control group. [Conclusions] Acupoint catgut embedding therapy can inhibit P53 protein expression, increase Bcl-2 protein expression and inhibit hippocampal neurons, thus reducing the cerebral damage induced by SE.