不同针刺法对P15在SAMP8不同脑区星型胶质细胞表达的影响

Effects of different acupuncture methods on P15 expression of astrocyte in different encephalic regions

关键词:  “醒脑开窍”针刺法  老年性痴呆(AD)  细胞周期因子(P15)  脑区  SAMP8

Key words:  "XNKQ" acupuncture method  Alzheimer’s disease(AD)  cell cycle factor(P15)  encephalic region  SAMP8

摘要:

[目的] 了解细胞周期相关因子细胞周期蛋白依赖性激酶抑制因子(P15)在老年性痴呆(AD)模型快速老化模型小白鼠(SAMP8)不同脑区星型胶质细胞中的表达规律及不同针刺组方(醒脑组和补虚组)对其表达的影响。[方法] 用免疫组化双标记法分别标记SAMP8脑组织星型胶质细胞(AS)和细胞周期蛋白依赖性激酶抑制因子(P15),用病理图像分析技术测定皮质、海马、纹状体、嗅球4个脑区中同时标记为胶质纤维酸性蛋白(GFAP)和P15阳性细胞数。[结果] 与正常组比较,AD模型组海马区同时表达GFAP和P15的阳性细胞数减少(P<0.05),其他3个脑区的表达差异无统计学意义。"醒脑组"可同时提高其在AD模型海马和嗅球中的表达,而"补髓组"只提高海马区的表达(P<0.05)。[结论] 8月龄SAMP8海马区表达P15的星形胶质细胞减少,可能是AD重要的病理机制;针刺可促进不同脑区星型胶质细胞P15的表达,并有针刺组方特异性。增加海马、嗅球区星形胶质细胞P15的表达,可能是"醒脑开窍"针刺法干预AD的潜在机制。

Abstract:

[Objective] To understand the expression regulation of cell cycle related factor P15(cyclin-dependent kinase inhibitor)of astrocyte in different encephalic regions in AD model mouse(SAMP8), and the effects of different acupuncture prescriptions(XNKQ prescription and reinforcing deficiency prescription) on it.[Methods] Astrocyte(GFAP) and P15 in SAMP8 brain were respectively labeled with immunohistochemistry double stain technique. By using pathologic image analysis technique, the positive cells both labeled wi GFAP and P15 were observed from four encephalic regions: cortex, hippocamp, striatum and bulbus cinereus.[Results] Compared with normal group, the quantity of positive cells reduced in hippocampus of AD model group. There was no statistically significant changes among other 3 encephalic regions. "XNKQ" group could raise the expression of positive cells in hippocamp and bulbus cinereus, and reinforcing deficiency group could only raise that of in hippocamp (P<0.05).[Conclusions] It may be an important pathogenesis of AD that the expression of P15 in astrocytes of hippocamp in 8-month age SAMP8 reduces. Acupuncture can promote the expression of P15 in astrocytes locating at different encephalic regions, and the target area is different for different acupuncture prescriptions. It can be potential mechanism of "XNKQ" acupuncture method on AD that P15 expression of astrocytes increases in hippocamp and bulbus cinereus.