载脂蛋白A1基因多态性与芪参益气滴丸疗效的关联性研究

Correlation between ApoA1 gene polymorphism and efficacy of Qishen Yiqi dripping pill

关键词:  载脂蛋白A1  单核苷酸多态性  芪参益气滴丸  气虚血瘀型冠心病

Key words:  Apolipoprotein A1(ApoA1)  single nucleotide polymorphism  Qishen Yiqi dripping pills  qi deficiency and blood stasis type of coronary heart disease

摘要:

[目的]探讨载脂蛋白A1(ApoA1)基因多态性与中药治疗气虚血瘀型冠心病之间的关系。[方法]符合诊断标准的气虚血瘀型冠心病患者42例,门诊服用芪参益气滴丸1个月,治疗前后对证候打分,各抽取血样1次,测定血脂、血糖、载脂蛋白含量。提取外周血白细胞DNA,聚合酶链反应(PCR)扩增ApoA1基因第一外显子,DNA测序检测多态性位点。[结果]ApoA1基因的第一外显子中的4个有义突变位点,对治疗前后的胸痛、胸闷、气短、神疲乏力、心悸、自汗、面色暗紫、总证候积分等证候的改变没有显著影响,对血糖、血脂、载脂蛋白含量的改变没有显著影响。[结论]ApoA1基因第一外显子的4个多态性位点对芪参益气滴丸治疗气虚血瘀型冠心病的疗效没有影响。

Abstract:

[Objective] To explore the relationship between single nucleotide polymorphism(SNP) of apolipoprotein A1(ApoA1) gene and efficacy of Qishen Yiqi(QSYQ) dripping pills on qi deficiency and blood stasis type of coronary heart disease.[Methods] Forty-two patients received QSYQ dripping pills for one month.The syndromes were scored,and blood samples were taken before and after treatment.The contents of blood fat,blood sugar and apoA and apoB were detected and the DNA was extracted from the leukocyte of the blood samples,and then the first extron of ApoA1 gene was amplified by polymerase chain reaction.The polymorphic site was detected by sequencing.[Results] Four significant mutant sites were found in ApoA1 gene.Between mutant groups and normal groups,the subtract values of deficiency of vital energy,lassitude,spontaneous perspiration,chest pain,suffocative sensation in the chest,palpitation,dark purple face and total syndrome score weren’t significantly different(P>0.05).The subtract values of blood glucose,triglyceride,and apolipoproteins weren’t also significantly different(P>0.05).[Conclusions] Four SNP sites in ApoA1 cann’t affect the efficacy of QSYQ dripping pills on qi deficiency and blood stasis type of coronary heart disease.