快速老化痴呆鼠海马,皮质p38表达的增龄变化及针刺影响的实验研究

Acupuncture effect on age-related change of p38 expression in hippocampus neocortex in SAMP10 Mice

关键词:  “益气调血,扶本培元”  SAMP10  蛋白杂交  皮质  海马  增龄变化  p38

Key words:  YiqiTiaoxue and FubenPeiyuan  age-accelerated Mouse(SAMP10)  Western blotting  neocortex  hippocampus  age-related change  p38

摘要:

[目的]探讨针刺的作用机制。[方法]采用western blotting技术,以快速老化痴呆鼠(SAMP10)为材料,同源正常老化小鼠(SAMR1)为对照,观察2、4、6、8、10、12月龄海马和皮质中p38表达的增龄变化,以及"益气调血,扶本培元"针法对8月龄SAMP10海马和皮质p38表达的影响。[结果]1)p38表达在正常老化R1海马和皮质均随增龄呈平稳上升趋势。8月龄后较2月龄存在明显差异。而P10则呈波动上升趋势。海马中4月龄即升高,6月龄达峰值后下降,10月龄至低谷但仍显著高于2月龄,此后至12月龄又上升;皮质中2月龄即升高,4月龄达峰值后下降,6月龄至低谷后再次升高并于8月龄达峰值,此后至12月龄再度下降,但仍显著高于2月龄水平。2)与8月龄R1相比,p38在P10海马和皮质表达普遍增高。"益气调血,扶本培元"针法具有腧穴特异性,可降低该蛋白在P10两个脑区的表达,使之接近R1的水平。[结论]1)p38表达在R1与P10两系相同脑区之间随增龄存在差异。2)p38的增龄变化与P10快速老化痴呆特征的时间段接近。3)"益气调血,扶本培元"针法可有效降低8月龄P10海马和皮质p38表达量。

Abstract:

[Objective] To explore the effect mechanism of acupuncture.[Methods] SAMP10 was used as model mouse and SAMR1 as normal control mouse.Western blotting technique was used to monitoring the difference of age-related p38 expression in neocortex and hippocampus between R1 and P10.The effect of YiqiTiaoxue and FubenPeiyuan acupuncture on the protein expression in 8-month old P10 brain was observed.[Results] The p38 expression had a stable age-related uprising tendency in the neocortex and hippocampus of R1 control.In P10,p38 expression exhibited an fluctuating age-related uprising tendency.These changes occurred at 4 to 6 month old and went on at the following months.In the hippocampus and neocortex of 8-month old P10,p38 expression exhibited a higher level than that of R1.The acupuncture of YiqiTiaoxue and FubenPeiyuan could reverse the difference mostly,the non-points group had no the same effect.[Conclusions] There is a difference in p38 expression in the same part of the brain between P10 and R1.The period of age-related changing is very approximate in the p38 expression and the onset of age-accelerated characters.The acupuncture of YiqiTiaoxue and FubenPeiyuan can effectively down-regulate the high level expression of p38 in 8-month old P10.