| 摘要: |
| [目的]观察滋水清肝饮对围绝经期抑郁模型大鼠下丘脑雌激素α受体mRNA(ER-αmRNA)表达及5-羟色胺(5-HT)含量的影响。[方法]将45只实验大鼠按体质量随机分为5组,每组9只。对照组(A);围绝经期抑郁症模型组(B);围绝经期抑郁症中药治疗组(C);围绝经期抑郁症雌二醇治疗组(D);围绝经期抑郁症雌二醇加百忧解治疗组(E)。A组行假手术,其余4组大鼠行卵巢摘除术+行慢性不可预见应激。第1轮刺激结束后开始给药。A、B组分别给予生理盐水2mL/d灌胃。C组给予滋水清肝饮(11.25g/kg)灌胃,1次/d;D组给予雌二醇(0.18 mg/kg)肌内注射,隔日1次;E组给予百忧解混悬液(1.8 mg/kg)灌胃,1次/d,加雌二醇肌内注射,隔日1次。给药21d。在末次治疗24 h后,颈椎脱臼法处死大鼠,冰盒上迅速分离下丘脑,采用逆转录-聚合酶联反应(RT-PCR)检测下丘脑ER-αmRNA表达,高效液相色谱—电化学检测器法测定下丘脑5-HT含量。[结果]模型组大鼠ER-αmRNA表达与对照组比较降低,有显著性差异(P<0.01),中药能升高大鼠ER-αmRNA的表达,且与模型组有显著性差异(P<0.05)。模型组大鼠5-HT水平降低与对照组比较有显著性差异(P<0.01)。中药治疗后,大鼠5-HT水平升高,且与对照组无显著性差异(P>0.05)。[结论]模型大鼠下丘脑中ER-αmRNA表达降低,同时可能还存在着5-HT功能低下。中药滋水清肝饮通过提高ER-αmRNA转录水平,使ER-α合成增加,提高E-ER的生物效应,进而可能影响及5-HT这一环节,升高脑内5-HT含量,从而降低围绝经期抑郁症的发生。 |
| 关键词: 围绝经期抑郁症 滋水清肝饮 下丘脑雌激素 5-羟色胺 |
| DOI：10.11656/j.issn.1672-1519.2008.02.39 |
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| Effect of Zishui Qinggan Decoction on hypothalamus ER-α mRNA expression and 5-HT content in rats with Depression in peri-menopausal period |
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JIANG Da-zhen,FENG Qiu-xia,GAO Ying-tang
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1.Korea;2.Tianjin Third Central Hospital, Tianjin 300170, China;3.The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To observe the effect of Zishui Qinggan decoction on hypothalamus ER-α mRNA expression and 5-HT content in rats with depression in peri-menopausal period.[Methods] Forty-five rats were randomly divided into five groups: control group,model group,TCM group,Estradiolum group and Estradiolum and Fluoxetine group.The rats of control group were treated with sham operation.The rats of other groups were treated with ovariosteresis and chronicity non-prediction stress.The rats of control and model group were administrated with physiologic saline and that of other group were individually administrated with Zishui Qinggan decoction,Estradiolum,Estradiolum and Fluoxetine.Hypothalamus ER-α mRNA expression and 5-HT content were determined by RT-PCR and HPLC-electrochemical detector method.[Results] The ER-α mRNA expression of model group was obviously lower than that of control group(P<0.01).The ER-α mRNA expression of TCM group was obviously higher than that of model group(P<0.05).The 5-HT of model group was obviously lower than that of control group(P<0.01) and The 5-HT of TCM group was obviously higher than that of control group(P>0.05).[Conclusions] The ER-α mRNA expression and 5-HT content exhibits low expression in rats of model group.Zishui Qinggan decoction may increase the content of 5-HT and increase the expression of ER-α mRNA and improve the condition of rats with depression in peri-menopausal period by enhancing ER-α mRNA transcriptional level and biological effect. |
| Key words: depression model in peri-menopausal period Zishui Qinggan decoction ER-α mRNA 5-HT |
