右归丸减轻甲状腺功能减退模型大鼠体内自由基过氧化损伤的机制探讨

Mechanism of Yougui pills in ameliorating the free radical peroxidation injury in Vivo in rats with hypothyroidism

关键词:  甲状腺功能减退症  SOD  右归丸

Key words:  Hypothyroidism  TCM therapy  SOD  Yougui pill

摘要:

[目的]探讨右归丸减轻甲状腺功能减退症模型大鼠体内自由基过氧化损伤的机制。[方法]采用丙基硫氧嘧啶灌胃给药造成实验性甲减大鼠模型,8周后处死大鼠,取血检测甲状腺功能,取血清测定超氧化物歧化酶(SOD)以及取甲状腺组织测定超氧化物歧化酶(SOD)的含量。[结果]甲减大鼠的血清SOD活力明显下降,治疗后右归丸、甲状腺片都可以显著提升大鼠低下的血清SOD活力,但其中以甲状腺片治疗效果最好。相关分析结果显示,大鼠血清SOD活力与三碘甲状腺原氨酸(T₃)、甲状腺素(T₄)、游离三碘状腺原氨酸(FT₃)、游离甲状腺素(FT₄)呈显著正相关(P<0.01),与促甲状腺激素(sTSH)呈显著负相关(P<0.05),即血清SOD活力随着甲状腺功能的改善而恢复正常。而甲状腺功能减退大鼠的甲状腺组织SOD活力明显过高,右归丸和甲状腺片均能改善过高的甲状腺组织SOD活力,其中以右归丸的效果最好。[结论]右归丸能提高甲状腺功能减退症模型大鼠的脂质过氧化应激反应能力、对减轻体内自由基过氧化损伤有一定作用。

Abstract:

[Objective] To investigate the mechanism of Yougui pills in lightening free radical peroxidative damage in rats with hypothyroidism. [Methods] Propylthiouracil intragastric dosage was adopted to result in experimental hypothyroidism in rat. Eight weeks later they were sacrificed and their blood was harvested to detect the function of thyroid gland and also the hepatocuprein (SOD) in blood serum. The thyroid gland tissue was obtained to determine the hepatocuprein (SOD) in it. [Results] The activity of SOD in hypothyroidism rats descended significantly. Yougui pills and the thyroid tablet up-regulated the activity of SOD significantly after treatment. The effectiveness of the thyroid tablet was better. Correlation analysis result displayed that the activity of SOD and T₃, T₄, FT₃, FT₄ were in significant positive correlation(P<0.01). Negative correlation was fund between the activity of SOD and TSH (P<0.05). But SOD in the glandular tissue of hypothyroidism rats was higher. Yougui pills and the thyroid tablet could improve the exorbitance of SOD to normal level. The effectiveness of Yougui pills was better. [Conclusions] Yougui pills can increase the reactive stress ability of lipid peroxidation in rats with hypothyroidism. It has some effect in decreasing the free radical peroxidation damage in the body.