大黄藿苓汤对腺嘌呤致慢性肾衰大鼠肾脏胶原Ⅰ、Ⅲ表达的影响

Effect of Da-huang Huo-ling decoction on expression of kidney collogen Ⅰ and Ⅲ in rats with chronic renal failure induced by adenine

关键词:  大黄藿苓汤  腺嘌呤  慢性肾功能衰竭  胶原Ⅰ  胶原Ⅲ

Key words:  Da-huang Huo-ling decoction  adenine  chronic renal failure  collagenⅠ  collagen Ⅲ

摘要:

[目的]探讨大黄藿苓汤对腺嘌呤致慢性肾功能衰竭(简称慢性肾衰)大鼠肾脏胶原Ⅰ、Ⅲ表达的影响。[方法]Wistar大鼠灌服腺嘌呤复制慢性肾衰大鼠模型,检测肾功能指标、肾脏组织病理学改变及胶原Ⅰ、Ⅲ表达,并观察大黄藿苓汤的影响。[结果]预防给药组、治疗给药组肾脏病理改变较模型组轻;血尿素氮、肌酐、尿酸均较模型组低(P<0.05);预防给药组与模型组相比胶原Ⅰ、Ⅲ表达均明显减少(P<0.05),治疗给药组与模型组相比胶原Ⅰ、Ⅲ表达无显著性差异。[结论]大黄藿苓汤对腺嘌呤致慢性肾功能衰竭大鼠肾脏有保护作用,且早期用药优于晚期用药。

Abstract:

[Objective] To explore the protective effect of Da-huang Huo-ling decoction on kidney collogen Ⅰand Ⅲ expression in rats with chronic renal failure induced by adenine. [Methods] The chronic renal failure was induced with adenine. They were divided into treatment medicated group and prevention medicated group. The kidney function, pathologic change of renal tissue and platelet derived growth factor (PDGF) expression, collagen Ⅰ and collagen Ⅲ expression were determined. The effect of Da-huang Huo-ling decoction on rats with chronic renal failure was observed. [Results] The pathologic changes of the treatment group and prevention group were less than that of the control group. Blood Urea Nitrogen (BUN), creatinine and uric acid were lower than that of model control group (P<0.05). The kidney collogen Ⅰand Ⅲ expression of prevention group was obviously reduced comparing with the control group(P<0.05). The kidney collagen Ⅰand Ⅲ expression of treatment group was not different obviously when compared with that of model control group(P>0.05). [Conclusions] Da-huang Huo-ling decoction has protective function against chronic renal failure in rat caused by adenine. Its effect mechanism may be restraining of the hyperplasia of kidney interstitial fibrous tissue by reducing the expression of kidney collagen Ⅰand Ⅲ.