不同年份柴胡种子SOD、POD活性与发芽率的测定

A test to SOD、POD activities and germination percentage of seeds of Bupleurum chinense in different years

关键词:  柴胡  超氧化歧化酶  过氧化物酶  发芽率

Key words:  Bupleurum chinense  SOD  POD  Germination percentage

摘要:

[目的]测定2003年、2004年、2005年3个不同年份的柴胡种子的超氧化歧化酶(SOD)、过氧化物酶(POD)活性与发芽率,并对两者的相关性进行探讨。[方法]运用氮蓝四唑(NBT)法和愈创木酚—过氧化氢比色法分别测定SOD、POD两种抗氧化酶活性,辅以传统发芽实验得到了3个不同年份的柴胡种子的发芽率。通过生物统计分析了实验指标间的的差异显著性和相关性。[结果]不同年份的柴胡种子两种抗氧化酶活性差异不显著(F_SOD=0.356<F_2,6,0.05;F_POD=4.675<F_2,6,0.05),发芽率有显著差异性(F=5.211,P<0.05)。SOD、POD酶活性与发芽率呈正相关但不显著(R_Y,1,2=0.0027<R_2,6,0.05)。[结论]柴胡种子的SOD、POD酶活性与发芽率均随贮藏时间的延长逐渐减弱,SOD、POD两种抗氧化酶活性与柴胡种子生命力的丧失有关,但不是影响柴胡种子生命力最关键的酶类。

Abstract:

[Objective] Detected the superoxide dismutase (SOD), peroxidase (POD) activities and the germination percentage of the seeds of Bupleurum chinense in different years. And also analyzed the relations between them. [Methods] Used NBT and Methoxyphenol-H₂O₂ colorimetry to mensurate SOD, POD activities and germination percentage was detected by traditional germination technique. And also analyzed the relations between them by SPSS biostatistics software. [Results] The activities of antioxidant enzymes varied unremarkably (F_SOD=0.356<F_2,6,0.05, F_POD=4.675<F_2,6,0.05)while the germination percentage was different evidently (F=5.211, ^*P<0.05)of the seeds of B. chinense in different years. They are positively correlated but unremarkable (R_Y,1,2=0.0027<R_2,6,0.05). [Conclusions] Both SOD, POD activities and germination percentage decreased with the store time of the seeds prolonged. The antioxidant enzymes related the seed vigor of B. chinense but they are not the most important enzymes to the seed vigor of B. chinense disappear or not.