功血宁Ⅱ号冲剂对诱发排卵大鼠卵巢白细胞介素-8表达的影响

Effect of GongxueningⅡon the expression of IL-8 in ovaries of rats with induced ovulation

关键词:  功血宁Ⅱ号冲剂  诱发排卵大鼠  卵巢  白介素8

Key words:  Gongxuening Ⅱ  induced ovulation rats  ovaries  IL-8

摘要:

[目的]观察功血宁Ⅱ号冲剂对诱发排卵大鼠卵巢白细胞介素-8(IL-8)表达的影响,探讨该药促进卵泡发育诱发排卵的作用机制。[方法]将未成年雌性大鼠随机分为模型对照组、阳性对照组和功血宁Ⅱ号高、低剂量组,分别给予蒸馏水、安坤赞育丸和功血宁Ⅱ号冲剂14d后,皮下注射妊马血清促性腺激素(PMSG)+人绒毛膜促性激素(hCG)制备诱发排卵的大鼠模型。分别在卵泡发育期和排卵前期处死动物摘取卵巢。常规制备卵巢切片,免疫组化法检测各组动物卵泡不同发育时期IL-8表达。[结果]功血宁Ⅱ号组在卵泡发育期和排卵前期IL-8的阳性细胞面积、阳性面积率、积分光密度、平均光密度均显著高于模型对照组(P<0.05或P<0.01),其中高剂量组的作用较低剂量组和阳性对照组显著。排卵前期各组的上述指标均明显高于相应卵泡发育期的各组(P<0.05或P<0.01)。[结论]功血宁Ⅱ号冲剂能够通过提高IL-8的表达,吸引和激活中性粒细胞,促进卵泡发育诱发排卵。

Abstract:

[Objective] To observe the effect of GongxueningⅡon the expression of ovaries IL-8 in rats with induced ovulation and explore the molecular mechanism in promoting follicular development and ovulation. [Methods] The female rats were randomly divided into model group, positive control group and GongxueningⅡhigh and low dosage group and they were individually administrated with distilled water, Ankunzanyu pills and GongxueningⅡfor fourteen days. The model of follicular development phase was established by injecting PMSG and the model of preovulatory phase was established by injecting HCG. The ovaries sections was prepared and determine the expression of ovaries IL-8 in rats with induced ovulation in diferent developmental phase immunohistochemically. [Results] The ovaries IL-8 of Gongxuening II group was obviously higher than that of model group on the area, area rate, IOD and MOD of folicular development phase and preovulatory phase (P<0.05 or P<0.01). The efect of Gongxuening II of high dosage group were obviously higher than that of low dosage and positive control group. The above indexes of follicular development phase was obviously higher than that of preovulatory phase in rats of every group (P<0.05 or P<0.01). [Conclusion] Gongxuening II can raise the expression of ovaries IL-8, attract and activate heterophil granulocyte and promote follicular development and ovulation.