加减补肺汤对COPD肺气虚证模型大鼠支气管肺泡灌洗液TNF-α、IL-8的影响

Effect of Jiajianbufei decoction on TNF-a and IL-8 of bronchoalveolar lavage fluid in COPD rats with deficiency of Lung-qi

关键词:  加减补肺汤  慢性阻塞性肺疾病  肺气虚证  TNF-α  IL-8

Key words:  Jiajianbufei decoction  chronic obstructive pulmonary diseases(COPD)  deficiency of lung-qi  TNF-a  IL-8

摘要:

[目的]研究加减补肺汤对慢性阻塞性肺疾病(COPD)肺气虚证模型对支气管肺泡灌洗液肿瘤坏死因子-α(TNF-α)、白介素细胞-8(IL-8)表达及大鼠气道病理改变的影响,探讨加减补肺汤治疗COPD肺气虚证的机制。[方法]用烟熏并复合木瓜蛋白酶雾化吸入法复制COPD"肺气虚证"大鼠模型,将100只雌雄各半大鼠随机分为5组:正常组、模型组、补高组、补低组、桂龙组。治疗后,光镜观察大鼠支气管、肺组织病理改变,放免法检测大鼠支气管肺泡灌洗液TNF-α、IL-8水平。[结果]与正常对照组比较,造模组支气管管腔及周围炎性细胞浸润,纤毛粘连、倒伏、脱落,肺泡扩大融合,数量减少;支气管肺泡灌洗液中TNF-α、IL-8高表达,各治疗组以上改变均明显减轻,以补高组最为显著(P<0.01)。[结论]加减补肺汤具有缓解气道炎症的作用,对COPD肺气虚证疗效确切。

Abstract:

[Objective] To study the effect of Jiajianbufei(modified decoction for tonifying the lung) decoction on TNF-a and IL-8 of bron-choalveolar lavage fluid in COPD rats with deficiency of lung-qi and to explore the mechanism of it in treating deficiency of lung-qi of COPD rats. [Methods] Deficiency of lung-qi of COPD in rats was established by using smudging and caroid aerosol rebreathing method. One hundred rats were randomly divided into five groups:normal group, model group, high dosage of Jiajianbufei decoction group, low dosage of Jiajianbufei decoction group, Guilong group. The changes of bronchus and lung tissue pathology were examined with light mi-croscope and the TNF-a and IL-8 level in bronchoalveolar lavage fluid was determined. [Results] Compared to normal control group, the inflammatory cell infiltration of bronchus lumina in model group was increased, the cilium conglutination, lodging and desquamation, alveolus expanding and quantity decreased. The TNF-a and IL-8 level of bronchoalveolar lavage fluid in model group were arisen as high expression. The change of treatment group was obviously improved and that of high dosage of Jiajianbufei decoction group was the most significant (P<0.01). [Conclusion] Jiajianbufei decoction may relief airway inflammation and has a definite curative effect on COPD rats with deficiency of lung-qi.