| 摘要: |
| [目的]测定不同来源胡黄连主根与须根中香草酸和肉桂酸含量。[方法]甲醇-0.1%磷酸(40:60)为流动相,TIANHE-C18(250nm×4.6nm,10μm)为色谱柱,检测波长260nm,流速:1mL/min,柱温25℃。[结果]香草酸、肉桂酸线性范围分别为0.72~72μg(r=0.9998)、0.35~3.50μg(r=0.9999),平均回收率分别为99.62%[平均标准偏差(RSD=0.75%)]、98.84%(RSD=1.65%)。胡黄连的主根与须根均含有香草酸与肉桂酸。[结论]该法快速、简便、精密度、重复性、稳定性好,适用于胡黄连含量分析测定。 |
| 关键词: 胡黄连 高效液相色谱法 香草酸 肉桂酸 |
| DOI：10.11656/j.issn.1672-1519.2008.06.33 |
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| Determination of vanillic acid and cinnamic acid in picrohiza scrophulariiflora pennell with HPLC |
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YAN Hai-hong, LI Bao-sheng, ZHANG Ya-qian
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Department of Pharmacy of Blood Disease Hospital of Chinese Academy of medical Sciences, Tianjin 300020, China
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| Abstract: |
| [Objective] To establish HPLC method for the determination of Vonillic acid and cinnamic acid in rhizoma and fibre of picro-hiza Scrophulariiflora Pennell.[Methods] The column was TianHe C18 (150 mm×4.6 mm) with 10 μm packing. The mobile phase used was methanol-phosphorus acid(40:60). The flow rate was 1.0mL·min-1, detection Wavelength was 260 nm. [Results] The linearity of this method was good in the ranges of 0.72-72 μg for Vonillic acid (r=0.999 8);0.35-3.50 μg for cinnamic acid (r=0.9999). The recoveries of Vonillic acid and cinnamic acid were 99.62% and 98.84%. RSD were 0.75% and 1.65% respectively. [Conclusion] The method is accu-rate, precise and suitable to determine Vonillic acid and cinnamic acid in Picrohiza Scrophulariiflora Pennell. |
| Key words: Picrohiza Scrophulariiflora Pennell HPLC Vonillic acid cinnamic acid |
