不同性别大鼠中药含药血清对成骨细胞增殖的影响

Influence of radix dipsaci containing serum on proliferation of osteoblasts in rats with different sex

关键词:  成骨细胞  含药血清  续断

Key words:  osteohlasts  radix despair containing serum  radix dipsaci

摘要:

[目的]观察不同性别大鼠的中药续断含药血清对成骨细胞增殖的影响。[方法]1)制备大鼠含药血清:取雌性与雄性Wistar大鼠各12只,按性别随机分为4组,分别为:雄性给药组,雄性对照组,雌性给药组,雌性对照组,每组各6只,给药组按体质量(4.5g/kg)分别给予续断水提液灌胃、对照组按体质量分别给予等量生理盐水,灌胃1周后取血,离心提取血清。2)分离、培养大鼠的原代成骨细胞。3)将各组大鼠血清稀释为2.5%,5%和10%3个浓度分别培养大鼠成骨样细胞,采用噻唑蓝法(MTT)检测细胞增殖能力。[结果]血清浓度为2.5%时,雌性给药组(0.5690±0.0295)与雄性给药组(0.3613±0.0983)比较,有显著性差异(P<0.05),雌性给药组与雌性对照组(0.3787±0.0476)比较,也有显著性差异(P<0.01);当血清浓度为5%时,雌性给药组(0.8793±0.1007)与雄性给药组(0.3560±0.0482)比较,有显著性差异(P<0.01),雌性给药组与雌性对照组(0.3863±0.0180)比较,也存在显著性差异(P<0.01);当血清浓度为10%时,雌性给药组(0.8560±0.0175)与雌性对照组(0.3817±0.1986)比较,存在显著性差异(P<0.05)。在各浓度的血清中,雄性给药组与雄性对照组之间均无显著性差异。[结论]大鼠的续断含药血清具有刺激成骨细胞增殖的作用。这种作用在雌性大鼠的血清中表达强于雄性大鼠。

Abstract:

[Objective] To observe the effect of Radix Dipsaci containing serum on the proliferation of osteoblasts in rats withdiscontinued administration and different sex.[Methods] The drug containing serum in rats was prepared.Twenty-four Wistar rats withequal male and female were randomly divided into four groups:male control group (Ⅰ), male drug-added group (Ⅱ), female control group (Ⅲ), female drug-added group (Ⅳ), Intragastric perfusion with Radix Dipsaci (4.5g crude drug/kg body weight) and saline respectivelyaccording to the animal body weight was given for one week and then the blood was gained.After centrifugation the supernatant fluid wasobtained.Then the osteoblasts were isolated and cultured.The proliferation of osteoblasts was analyzed quantitatively.The cells weredisposed and added with different concentration of rat serum, and divided into 2.5%, 5% and 10% Radix Dipsaci group.The proliferationof cells was tested with MTT.[Results] When the medium concentration was 2.5% of Radix Dipsaci significant difference was foundbetween male and female drug-added group (P<0.05).There was also significant difference between female drug-added and femaleantitheses group when the medium was 5% concentration of Radix Dipsaci rat serum (P<0.01).While the medium was 10% concentrationof Radix Dipsaci significant difference could be seen between female drug-added group and female antitheses group (P<0.05).However,no matter the concentration of Radix Dipsaci was, no significant differences could be seen between male drug-added group and malecontrol group.[Conclusion] Radix Dipsaci can stimulate the proliferation of osteoblasts.This function in female drug-added group wasmore significant than that of male drug added group.