益气活血方对肺纤维化黏附分子表达影响的实验研究

Effect of supplementing Qi and activating blood prescription on the expression of adhesion molecule in pulmonary interstitial fibrosis

关键词:  益气活血方  黏附分子  肺纤维化  细胞间黏附分子-1  血管细胞间黏附分子-1

Key words:  supplementing Qi and activating blood prescription  adhesion molecule  pulmonary interstitial fibrosis  ICAM-1  VCAM-1

摘要:

[目的]通过观察中药益气活血方对博莱霉素肺纤维化大鼠肺组织黏附分子[细胞间黏附分子-1(ICAM-1)、血管细胞间黏附分子-1(VCAM-1)]的影响,进一步探讨中药益气活血方治疗肺纤维化的机制。[方法]采用经典的博莱霉素肺纤维化模型,将80只符合实验标准的大鼠随机分为4个组,即正常对照组、模型组、泼尼松组、益气活血方组,镜下观察各组病理形态学表现及肺组织炎症积分的情况,并采用免疫组化法观察各组肺组织黏附分子ICAM-1、VCAM-1的表达,将结果进行统计学分析。[结果]益气活血方能减轻博莱霉素肺纤维化大鼠肺泡炎程度;益气活血方能下调黏附分子ICAM-1、VCAM-1在肺组织的表达。[结论]中药益气活血方能下调黏附分子ICAM-1、VCAM-1在肺组织的表达,这可能是其治疗防治肺纤维化的机制之一。

Abstract:

[Objective] To observe the effect of supplementing Qi and activating blood on the expression of lung tissue ICAM-1 andVCAM-1 in rats with pulmonary interstitial fibrosis induced by bleomycin and explore its effect mechanisms.[Methods] Eighty rats withpulmonary interstitial fibrosis induced by bleomycin were randomly divided into four groups.They were normal control group, modelgroup, prednisone group and Chinese medicine group.The condition of the pathologic changes and lung’s tissue inflammation integralwere observed.Immunohistochemical method was used for detecting the expressions of lung tissue ICAM-1 and VCAM-1 in each group.Then the results were analyzed statistically.[Results] Supplementing Qi and activating blood prescription could reduce pulmonary alveolitis of the rat with bleomycin-induced pulmonary interstitial fibrosis.It could inhibit the expression of the ICAM-1 and VCAM-1 in thelung tissue.[Conclusion] Supplementing Qi and activating blood prescription can inhibit the expression of the ICAM-1 and VCAM-1 inthe lung tissue.This might be one of the mechanisms of preventing and curing the pulmonary interstitial fibrosis.