速效救心丸对动脉粥样硬化大鼠SDF-1/CXCR4表达的影响

Effect of Suxiao Jiuxin pellet on the expression of SDF-1/CXCR4 in atherosclerosis rats

关键词:  速效救心丸  动脉粥样硬化  基质细胞衍生因子  趋化因子受体

Key words:  Suxiao Jiuxin Pellet  atherosclerosis  SDF-1  CXCR4

摘要:

[目的]研究速效救心丸对动脉粥样硬化(AS)大鼠模型基质细胞衍生因子(SDF-1)/趋化因子受体(CXCR4)表达的影响进而探讨其防治AS的机制。[方法]采用高胆固醇饮食加大剂量维生素D3腹腔一次性注射方法建立AS大鼠模型。60只SD大鼠随机分为6组:正常组,模型组,速效救心丸低、中、高剂量组,阿托伐他汀组。12周后取胸主动脉苏木—伊红(HE)、三色(MASSON)染色,用图像分析系统分析其病理改变,免疫组化法检测SDF-1及CXCR4蛋白表达情况。[结果]与模型组相比,速效救心丸中、高剂量组及阿托伐他汀组可明显减轻动脉内膜及中膜厚度,中药高剂量组还可抑制胶原纤维增殖,并降低胶原纤维在血管壁中的比例;各中药组及西药组均可下调SDF-1/CXCR4蛋白的表达。[结论]速效救心丸可明显拮抗AS大鼠病变动脉内膜及中膜增生,抑制胶原纤维增殖,从而延缓AS进程,其机制可能与抑制SDF-1/CXCR4轴功能有关。

Abstract:

[Objective] To investigate the effect of Suxiao Jiuxin(improving the heart function with a high speed) Pellet on the expression of SDF-1/CXCR4 in rats with atherosclerosis and to find it’s mechanism of anti-atherosclerosis. [Methods] Atherosclerosis was established in rats by feeding with atherogenic diet and intraperitoneal injecting with vitamin D3 as a bolus. Sixty SD rats were randomly divid-ed into 6 groups:normal group(N),AS model group(Mo),Suxiao Jiuxin pellet low(SXL),medium(SXM),high(SXH) dosage group and Astorvastain group(ATO). Twelve weeks later,the pathological change of Aortas was analyzed by Image Pro Plus system;SDF-1 and CXCR4 were examined by immunohistochemistry. [Results] Compared with Mo,the SXM,SXH and ATO could significantly reduce the arterial intima and media thickness,SMH could also inhibit the proliferation of the collagen fiber and decrease percentage in vessel wall;SXL,SXH,SXN and ATO could decrease the expression of SDF-1 and CXCR4;Between ATO,SXM and SXH there were no significant difference(P>0.05). [Conclusion] Suxiao Jiuxin Pellet(medium,high dosage) can prevent the proceeding of AS by reducing the arterial thickness,inhibiting proliferation of the collagen fiber,delaying the process of AS.