针刺对2型糖尿病大鼠弓状核INSR基因表达的影响

Effect of acupuncture on nucleus arcuatus INSR mRNA expression in rats with type 2 diabetes mellitus

关键词:  针刺  弓状核INSR基因  2型糖尿病  原位杂交  优降糖

Key words:  acupuncture  nucleus arcuatus INSR mRNA  type 2 diabetes mellitus  hybridization in situ  Glibenclamide

摘要:

[目的]探讨2型糖尿病(T2DM)大鼠是否存在中枢胰岛素抵抗(IR)以及针刺对T2DM大鼠弓状核胰岛素受体(INSR)基因表达的影响。[方法]给食源性肥胖大鼠腹腔注射小剂量链脲佐菌素(STZ)造成T2DM模型,随机分为针刺组、优降糖组、模型组,设正常对照组。处理4周后,用罗氏活力型血糖仪测空腹血糖(FBS)、放免法测空腹胰岛素(FINS)、原位杂交测弓状核INSRmRNA表达。[结果]治疗后针刺组FBS、FINS显著降低,与模型组差异显著,接近正常组水平;优降糖组FBS显著降低,接近正常组水平(P>0.05),FINS低于模型组但无显著差异(P>0.05)。模型组弓状核INSR基因表达明显低于针刺组(P<0.01)和正常组(P<0.05),低于优降糖组但无显著差异(P>0.05);针刺组、优降糖组与正常组比较无显著差异(P均>0.05);优降糖组明显低于针刺组(P<0.05)。[结论]T2DM大鼠存在弓状核INSR基因表达低下,T2DM机体存在中枢IR,针刺和优降糖均可以改善T2DM大鼠弓状核INSR基因的表达,而针刺对改善T2DM大鼠弓状核INSR基因表达的作用优于优降糖。

Abstract:

[Objective] To study if the rats with Type 2 Diabetes Mellitus exist center insulin resistance and the effect of acupuncture on the expression of insulin receptor gene(INSR) of nucleus arcuatus in rats with Type 2 Diabetes Mellitus. [Methods] Obese rats due to diet were injected i.p. with small dose streptozotocin to induce type 2 diabetes mellitus. Then the rats were randomly divided into acupuncture, Glibenclamide and model group. After treatment for 4 weeks, FBS was examined with active blood sugar meter, FINS examined by radioimmunoassay, INSR mRNA expression of nucleus arcuatus were evaluated with hybridization in situ. [Results] Serum levels of FBS (15.79±1.87) and FINS (37.20±3.92) in model group were higher than that of control group (5.16±0.13, 23.57±1.63), amount of nucleus arcuatus positive cell (0.0416±0.0153) was significantly depressed compared with control group (0.1194±0.0242). Serum levels of FBS (5.46±0.20) and FINS(20.87±1.80) in acupuncture group were obviously lower than that of model group, amount of nucleus arcuatus positive cell (0.1524±0.0198) in acupuncture group was enhanced compared with model, which was similar to control. FBS (5.26±0.13) in Glibenclamide groups was reduced obviously compared with model, serum levels of FINS (28.10±1.86) and amount of nucleus arcuatus positive cell (0.0818±0.0234) in Glibenclamide group was improved when compared with model, but no significant differences were found between them. [Conclusion] Rats with Type 2 Diabetes Mellitus exists a depressed nucleus arcuatus INSR mRNA expression. Acupuncture and Glibenclamide can improve its expression of nucleus arcuatus in rats with Type 2 Diabetes Mellitus, but the effect of acupuncture was better than that of Glibenclamide.