针刺对心肺复苏后家兔心肌ATP酶活性及SOD、MDA的影响

Effects of acupuncture on ATPase activity and superoxide dismutase, malondialdehyde content in rabbit cardiac muscle after cardiopulmonary resuscitation

关键词:  心肺复苏  针刺  三磷酸腺苷酶  超氧化物歧化酶  丙二醛

Key words:  cardiopulmonary resuscitation  acupuncture  ATPase  superoxide dismutase (SOD)  malondialdehyde (MDA)

摘要:

[目的]探讨针刺对心肺复苏后家兔心肌细胞三磷酸腺苷(ATP)酶活性、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响。[方法]30只家兔随机分3组,假手术(Sham)组不进行窒息和复苏;常规复苏组心搏骤停后予常规心肺复苏术;针刺复苏组心搏骤停后予常规心肺复苏术并针刺人中、内关穴。所有动物于自主循环恢复后2h取心肌组织,测定ATP酶活性、SOD活性及MDA含量。[结论]与Sham组相比,针刺复苏组、常规复苏组心肌ATP酶活性降低(P<0.01);与常规复苏组相比,针刺复苏组ATP酶活性升高(P<0.05)。复苏后两组SOD活性均低于Sham组(P<0.01),MDA含量均高于Sham组(P<0.01);针刺复苏组SOD活性高于常规复苏组(P<0.05),而MDA含量低于常规复苏组(P<0.05)。[结论]针刺人中、内关穴可增加心肺复苏后心肌组织ATP酶活性,增加SOD活力,减少MDA生成。

Abstract:

[Objective] To investigate the effects of acupuncture on Renzhong, Neiguan point on activity of ATPase, superoxide dismutase (SOD), malondialdehyde (MDA) in rabbit cardiac muscle after cardiopulmonary resuscitation. [Methods] Thirty rabbits were randomly divided into 3 groups (n=10), the sham group only took pretreatment but asphyxia cardiac arrest was not induced; the normal CPR (cardiopulmonary resuscitation) group were given normal CPR after cardiac arrest; the acupuncture and CPR group were given with normal CPR and acupuncture on Renzhong, Neiguan point. Two hours after the returning of the spontaneous circulation (ROSC), pieces of myocardium tissue for ATP and SOD, MDA content testing were obtained from all the animals. [Results] ATPase activity were decreased significantly both in the normal CPR group and the acupuncture and CPR group compared with that of sham group (P<0.01), but ATPase activity were higher in the acupuncture and CPR group than that in the normal CPR group (P<0.05). Meanwhile SOD activity decreased and MDA contents increased significantly after resuscitation compared with sham group (P<0.01); SOD and MDA in the acupuncture and CPR group were higher and lower than that in the normal CPR group respectively (P<0.05).[Conclusion] Acupuncture on Renzhong, Neiguan point may increase ATPase activity, superoxide dismutase activity, and decrease malondialdehyde content in rabbit cardiac muscle after cardiopulmonary resuscitation.