不同季节的葎草中木犀草素-7-O-β-D-葡萄糖苷和大波斯菊苷的含量测定

Determination of Luteolin-7-O-β-D-glucoside and cosmosiin in Humulus scandens in different seasons

关键词:  葎草  木犀草素-7-O-β-D-葡萄糖苷  大波斯菊苷  含量测定  季节

Key words:  Humulus scandens  Luteolin-7-O-β-D-glucoside  cosmosiin,content determination  season

摘要:

[目的]同时测定葎草中木犀草素-7-O-β-D-葡萄糖苷和大波斯菊苷的含量。[方法]采用高效液相色谱法(HPLC)测定葎草中木犀草素-7-O-β-D-葡萄糖苷和大波斯菊苷的含量,色谱柱kromasilC₁₈,以甲醇-0.2%磷酸溶液(45:55)为流动相,流速为1.0mL/min,柱温35℃,检测波长为350nm。[结果]木犀草素-7-O-β-D-葡萄糖苷在0.072～0.36μg的范围内与峰面积积分值成良好的线性关系(r=0.9999),精密度相对平均标准差(RSD)=2.06%,平均加样回收率为99.3%,RSD为2.8%;大波斯菊苷在0.05～0.40μg的范围内与峰面积积分值成良好的线性关系(r=0.9997),精密度RSD=1.73%,平均加样回收率为100.2%,RSD%为2.7%。葎草中木犀草素-7-O-β-D-葡萄糖苷的含量在7、8月份含量最高,大波斯菊苷在6月份含量最高。[结论]该方法简单、灵敏、稳定、可靠,可用于葎草中木犀草素-7-O-β-D-葡萄糖苷和大波斯菊苷的含量测定。葎草适宜的采收季节应在6～8月份。

Abstract:

[Objective] To determine the content of Luteolin-7-O-β-D-glucoside and cosmosiin in Humulus scandens.[Methods] The content of humulus by Soxhlet was extracted and using high-performance liquid chromatography (HPLC) Humulus scandens in Luteolin7-O-β-D-glucoside and cosmosiin content were determined.The column was kromasilC18 and the mobile phase was methanol-0.2% phosphoric acid (45:55),the flow rate was set at 1.0 mL/min,the column temperature was 35 ℃,and the UV detector was set at 350 nm.[Results] Luteolin-7-O-β-D-glucoside content showed a good linear relationship within 0.05～0.40 μg (r=0.9997),the precision RSD= 2.06%,and the average recovery was 99.3%,RSD was 2.8%;cosmosiin content showed a good linear relationship within 0.072～0.36 μg (r=0.999 9),the precision RSD=1.73%,and the average recovery was 100.2%,RSD was 2.7%.The content of Luteolin-7-O-β-D-glucoside was the highest in July and August,and the cosmos was the highest in June.[Conclusion] The method is simple,sensitive,stable,reliable,and it can be used in the determination of Humulus scandens luteolin-7-O-β-D-glucosidase and the cosmos by HPLC.The appropriate harvesting season of Humulus scandens is from June to August.