鼻康喷雾剂的抗炎作用研究

Anti-inflammatory effect of spraying agent for nasal healing

关键词:  鼻康喷雾剂  小鼠耳廓致炎实验  大鼠蛋清足跖肿胀实验  大鼠棉球肉芽肿实验  抗炎

Key words:  Nasal Healing Spraying Agent(NHSA)  the model of auricular swelling reaction in mice  the rat paw edema model reaction  the Cotton Ball Granuloma reaction in rat  anti-inflammatory

摘要:

[目的]探讨鼻康喷雾剂的抗炎作用。[方法]采用小鼠耳廓致炎实验、大鼠蛋清足跖肿胀实验和大鼠棉球肉芽肿实验,设阳性和空白对照。[结果]鼻康喷雾剂空白对照组小鼠耳廓肿胀度(12.05±1.52)mg,鼻康喷雾剂大、中、小剂量组的小鼠耳廓肿胀度分别为(3.42±3.32)mg、(5.95±2.04)mg、(10.30±1.75)mg,与空白对照组比较有统计学意义(P<0.05或P<0.01);鼻康喷雾剂大、中、小剂量组在致炎后2h大鼠右后足跖肿胀度明显减小,4～6h时作用最为明显,与空白对照组比较有统计学意义(P<0.05或P<0.01);空白对照组大鼠肉芽肿质量为(224.99±83.09)mg/100g体质量,鼻康喷雾剂大、中、小剂量组大鼠肉芽肿质量分别为(144.61±25.82)mg/100g体质量、(163.78±21.49)mg/100g体质量、(210.42±69.60)mg/100g体质量,与空白对照组比较明显减小(P<0.05)。[结论]鼻康喷雾剂能够减轻二甲苯所致的小鼠耳廓肿胀度、减轻蛋清所致的大鼠右后足跖肿胀度、对大鼠棉球肉芽组织增生有抑制作用,有抗炎作用。

Abstract:

[Objective] To study the anti-inflammatory effect of Nasal Healing Spraying Agent(NHSA). [Methods] We used the model of auricular swelling reaction in mice,the rat paw edema model reaction,and the Cotton Ball Granuloma reaction in rat.The animals were designed into blank and positive control groups. [Results] The degree of swelling of mice auricle of blank control group was 12.05±1.52 mg;The degrees of the high dose group,middle dose group,low dose group were 3.42±3.32 mg,5.95±2.04 mg,10.30±1.75 mg respectively,and were significant in statistic analysis(P<0.05 or P<0.01);The rate of the right postal toes and ankle swelling of rat of the high dose group,middle dose group,low dose group were remarkably decreased 2 h after making inflammation,while the most significant was found at 4～6 h.They were statistically significant difference(P<0.05 or P<0.01) compared with the blank control group;The weight of granuloma in rats from the blank control group was 224.99 ±83.09 mg/100g weight;The weights of the high dose group,middle dose group,low dose group are 144.61±25.82 mg/100 g body weight,163.78±21.49 mg/100 g b.w.,210.42±69.60 mg/100 g b.w.respectively,and were significant statistically(P<0.05). [Conclusion] NHSA could abate the degree of swelling of mice auricle and had anti-inflammatory effect.It could abate the rate of the right postal toes and ankle swelling of rat and had anti-inflammatory effect.It could inhibit the formation of cotton ball granuloma in rats and had anti-inflammatory effect.NHSA had immunoregulatory function.