脑康Ⅱ号对糖尿病大鼠血清中甲基乙二醛含量的影响

Effects of Naokang Ⅱ on concentration of methylglyoxal in serum of rats with diabetes

关键词:  脑康Ⅱ号  甲基乙二醛  糖尿病  气相色谱-质谱联用

Key words:  Naokang Ⅱ  methylglyoxal  diabetes  gas chromatography-mass spectrometry(GC-MS)

摘要:

[目的]观察脑康Ⅱ号对糖尿病大鼠血清甲基乙二醛含量的影响,以期为该药用于临床治疗糖尿病认知障碍提供实验依据。[方法]高糖高脂饮食结合腹腔注射链脲佐菌素诱发糖尿病大鼠模型,并用不同剂量的脑康Ⅱ号(分别含生药0.5、1.0、2.0 kg/L)治疗4周。采用气相色谱-质谱联用仪(GC-MS)测定糖尿病大鼠血清中甲基二乙醛(MG)的含量。[结果]与对照组相比,模型组大鼠血清中MG含量明显升高,应用低、中、高剂量的脑康Ⅱ号治疗后,MG的表达水平均下降。[结论]脑康Ⅱ号可显著下调糖尿病大鼠血清内增高的MG水平,这可能是其治疗糖尿病认知障碍的机制之一。

Abstract:

[Objective] To observe the effect of Naokang Ⅱ on the concentration of methylglyoxal(MG) in serum of diabetic rats in order to provide the theoretical basis for its clinic application in treating cognitive dysfunction in diabetic patients.[Methods] The diabetes mellitus(DM) was produced in rats by intraperitoneal injection of streptozotocin and feeding with high fat food and sucrose diet.The Naokang II-treated rats were given different doses of Naokang Ⅱ per oral,whereas the control and DM model groups were given double distilled water for 4 consecutive weeks.Gas Chromatography-Mass Spectrometry(GC-MS) was applied to determine the concentration of methylglyoxal(MG) in serum of rats.[Results] The concentration levels of MG in diabetic rats(216.67±15.56) μg/L was significantly high-er than that in the control group(85.29±24.97) μg/L.Different doses of Naokang Ⅱ could restore the level of MG to(178.8±19.34) μg/L,(137.9±16.95)μg/L and(128.47±16.77) μg/L,respectively.[Conclusion] Naokang Ⅱ can down-regulate the level of MG in serum of diabetic rats,and this effect may be one of the mechanisms in treating diabetic cognitive dysfunction.