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| 丹参酮B钠盐对局灶性脑缺血/再灌注损伤大鼠海马神经递质含量的影响 |
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蔡青1, 张伯礼2, 黄淑芸2, 汪涛2, 谭俊珍1, 周涛1, 李春深1
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1.天津中医药大学中医学院, 天津, 300193;2.天津中医药大学, 天津, 300193
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| 摘要: |
| [目的]观察大鼠脑缺血/再灌注(I/R)损伤后海马氨基酸类神经递质含量变化及丹参酮B钠盐对其的影响,探讨丹参酮治疗脑缺血可能的作用机制。[方法]采用栓线法制备局灶性脑缺血再灌注动物模型,将Wistar大鼠随机分为伪手术组(Sham)、模型组(I/R)、丹参酮B钠盐低、中、高剂量组对大鼠进行实验观察。应用高效液相方法检测各组大鼠海马谷氨酸、γ-氨基丁酸(GABA)含量。[结果]高效液相色谱(HPLC)结果显示:与模型组比较丹参酮B钠盐各组可不同程度降低海马谷氨酸、GABA含量。[结论]丹参酮B钠盐通过降低兴奋性神经递质谷氨酸而对抗脑缺血再灌注损伤。 |
| 关键词: 缺血再灌注损伤 谷氨酸 高效液相 γ-氨基丁酸 海马 |
| DOI：10.11656/j.issn.1672-1519.2010.06.23 |
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| 基金项目:天津市高等学校科技发展基金计划项目(No20070301) |
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| Effect of sodium tanshinone B on content of glutamate acid and GABA in hippocampus of rats following focal ischemia and reperfusion injury |
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CAI Qing1, ZHANG Bo-li2, HUANG Shu-yun2
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1.The Traditional Chinese Medical College of Tianjin University of TCM, Tianjin 300193, China;2.Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To investigate the effects of sodium Tanshinone B on the content of glutamate acid(Glu),γ-aminobutyric acid(GABA) in the hippocampus of rat following focal ischemia-reperfusion injury.[Methods] The rat model of focal cerebral ischemia-reperfusion was established with middle cerebral artery occlusion(MCAO) by reversibly inserting a nylon thread.The rats were randomized into sham operated group,model group and sodium Tanshinone B groups.The concentrations of Glutamate and GABA were analyzed with HPLC.[Results] The levels of glutamate and GABA were significantly lower in each sodium Tanshinone group than that of model group.[Conclusion] The results showed that pretreatment with sodium Tan B could significantly decrease the releasing of glutamate and GABA after ischemia-reperfusion injury. |
| Key words: Ischemia-Reperfusion injury glutamate acid HPLC GABA hippocampus |
