痹胶囊对实验性骨关节炎大鼠MMP-3和TIMP-1的影响

Effect of Biqi capsule on the MMP-3 and TIMP-1 in rats with osteoarthritic

关键词:  骨关节炎  痹褀胶囊  血清基质金属蛋白酶-3  基质金属蛋白抑制酶-1

Key words:  osteoarthritis  Biqi Capsule  MMP-3  TIMP-1

摘要:

[目的]观察痹褀胶囊对实验性骨关节炎(OA)大鼠软骨、关节液及血清基质金属蛋白酶-3(MMP-3)、基质金属蛋白抑制酶-1(TIMP-1)的影响,探讨痹褀胶囊保护关节软骨的作用机制。[方法]选Wistar大鼠,木瓜蛋白酶膝关节注射造模,痹祺胶囊灌胃治疗8周,酶联免疫吸附(ELISA)法检测软骨、关节液及血清MMP-3、TIMP-1的活性。[结果]痹祺胶囊能提高模鼠软骨TIMP-1的活性(P<0.01),降低关节液MMP-3的活性,使软骨、关节液MMP-3/TIMP-1的比值均降低(P<0.01);对血清MMP-3、TIMP-1及MMP-3/TIMP-1比值无明显影响(P>0.05)。[结论]痹褀胶囊保护关节软骨的作用可能是通过提高软骨TIMP-1的活性,降低关节液MMP-3的活性,使软骨、关节液MMP-3/TIMP-1的比值减小,从而抑制MMP-3对关节软骨基质蛋白多糖的裂解活性而起保护作用的。

Abstract:

[Objective] To observe the effect of Biqi Capsule on the MMP-3 and TIMP-1 of serum,synovia and cartilage matrix in rats with Osteoarthritis and to explore its mechanism in protecting cartilage matrix.[Methods] In Wistar rats,4% papain was injected into their knee joints to establish the animal model of osteoarthritis.They were treated with Biqi Capsule through intragastric administration for eight weeks and were killed at the end of experiment.The contents of MMP-3 and TIMP-1 in the Serum,synovia and cartilage were tested by ELISA analysis.[Results] Biqi Capsule could enhance the TIMP-1 activity in cartilage matrix,inhibit the MMP-3 activity in synovia(P<0.01),and decrease MMP-3/TIMP-1 ratio in cartilage matrix,synovia(P<0.01).But it had no effect to the expression of MMP-3,TIMP-1 and MMP-3/TIMP-1 ratio in Serum(P>0.05).[Conclusion] The mechanism of Biqi Capsule’s in protecting cartilage matrixm may be inhibiting degradation of proteoglycans in cartilage matrix by enhancing the TIMP-1 activity in cartilage matrix,inhibiting the MMP-3 activity in synovia,decreasing the ratio of MMP-3/TIMP-1 in cartilage matrix,synovial.