调气消积汤对Lewis肺肿瘤小鼠抑瘤作用及细胞凋亡影响的实验研究

Effects of the Tiaoqixiaoji decoction on anti-tumor and apoptosis in mice with Lewis lung cancer

关键词:  调气消积汤  Lewis肺肿瘤  细胞凋亡

Key words:  Tiaoqixiaoji decoction  Lewis lung cancer  apoptosis

摘要:

[目的]探讨调气消积汤对Lewis肺肿瘤的抑瘤作用及细胞凋亡的影响。[方法]C57BL/6小鼠常规接种Lewis肺肿瘤,随机分为:荷瘤对照组(MG)、调气消积汤组(TG)、顺铂组(DG)、综合组(ZG),每组10只。每天分别予饮用水、调气消积汤各0.4 mL灌胃,共12 d;隔日1次腹腔注射生理盐水、顺铂0.13 mL共3 d。观察其日常生活状况和肿瘤生长情况,应用原位末端转移酶标记技术(TUNEL)检测调气消积汤对Lewis肺肿瘤细胞凋亡的影响。[结果]调气消积汤能改善Lewis肺肿瘤小鼠一般状况,与荷瘤对照组比差异有统计学意义(P<0.01)。各治疗组的瘤质量均低于荷瘤对照组,差异显著(P<0.01)。光镜下观察,各治疗组与荷瘤对照组比较,异型细胞较少,染色质浓缩边集,病理性核分裂较少。TUNEL法结果显示,各治疗组与荷瘤对照组相比凋亡细胞明显增多,绿色荧光强度高;尤以综合组增多最为明显。[结论]调气消积汤可以改善荷瘤小鼠的一般生活状况,对Lewis肺肿瘤有抑制作用,能促进肿瘤细胞凋亡。

Abstract:

[Objective] To explore the therapeutic effect of Tiaoqixiaoji(TQXJ) decoction on anti-tumor effect and apoptosis of Lewis lung cancer-bearing mice.[Methods] C57BL/6 mice were inoculated with Lewis lung cancer cells routinely.Then they were randomly divided into: model group(MG),TQXJ group(TG),DDP(Cisplatin) group(DG) and TQXJ-DDP group(ZG),with 10 mice in each group.Different drugs were given to each group in order to observe the daily life and the tumor growing status of the mice.Determination of the cell apoptosis in the tumorous tissue was performed by TUNEL method.[Results] The general status of the Lewis tumor-bearing mice could be improved by TQXJ Decoction compared with MG group(P<0.01).The tumor weight of each treatment group was lighter than the MG(P<0.01).There were some cell morphological changes of the TG,DG and ZG,including the chromatin condensed in margin and the pathological karyokinesis reduced obviously compared with the MG.In situ TUNEL assay showed that increased apoptotic cells could be seen in all treatment groups;the green fluorescence intensity was high and the ZG was highest.[Conclusion] TQXJ Decoction is effective in improving the general life status of tumor-bearing mice,and it can inhibit the growth of the Lewis lung cancer,and also can induce the apoptosis of the Lewis lung cancer cells.