蝎毒多肽抑制白血病干细胞龛内活化的实验研究

Experiment research on PESV for inhibiting activation of leucemia stem cell in the niche

关键词:  蝎毒多肽  白血病干细胞  龛  活化效应

Key words:  PESV  LSC  niche  activating effect

摘要:

[目的]观察蝎毒多肽(PESV)抑制白血病干细胞(LSC)在骨髓龛内的活化效应。[方法]分离白血病/急性淋巴细胞白血病(AML/ALL)患者LSC,置于Transwell小室中,分为高中低剂量组及对照组,分别加入不同浓度蝎毒多肽后培养,计算不同浓度组及不同时间段的LSC迁移率。[结果]PESV组迁移率分别为(25.01±6.83)%、(36.85±3.83)%、(50.73±7.15)%,与对照组(49.10±6.12)%比较差异具有显著性(P<0.05),高剂量组迁移率高于中低剂量组。[结论]不同浓度PESV均有抑制白血病干细胞活化作用,抑制强度呈浓度依赖。

Abstract:

[Objective] To observe the effect of PESV(peptide extract from scorpion venom) for inhibiting the activation of leucemia stem cells(LSC) in the niche.[Methods] LSC were isolated from patients with AML/ALL and stored in the Trans well.Then they were divided into four groups: high-dose group,middle-dose group,low-dose group and the positive control group.After cultured with different concentrations of PESV,the transport ratios of LSC in various concentration groups were computed at different time.[Results] The transport ratios cultured with different concentrations of PESV were(25.01±6.83)%,(36.85±3.83)%,(50.73±7.15)%,and there was a significant difference compared with that of the positive control group(49.10±6.12)%(P<0.05).The transport ratio in the high-dose group is more than that of the middle and lose-dose groups.[Conclusion] All different concentrations of PESV have the inhibiting effects against the activation of LSC with a inhibiting intensity depending on the concentrations of PESV.