慢性应激对实验性2型糖尿病倾向大鼠血浆去甲肾上腺素、肾上腺素含量影响的实验研究

Influence of stress stimulation on serum epinephrine and norepinephrine in rats with experimental type 2 diabetes mellitus tendency

关键词:  慢性应激  糖尿病  去甲肾上腺素  肾上腺素

Key words:  emotion stimulation  diabetes mellitus  epinephrine  norepinephrine

摘要:

[目的]从糖尿病多因素致病角度出发,探讨情志因素对2型糖尿病发病的作用机制———气机失调证,以及具有理气降逆散结功效的复方中药心身5号方的调节作用。[方法]采用旋转、拥挤、限制3种情志刺激结合腹腔注射少量链脲佐菌素(STZ)的实验方法,制作了2型糖尿病倾向大鼠模型,实验动物分为正常组、应激组、STZ组、应激+STZ组和中药组。检测血浆去甲肾上腺素(NE)、肾上腺素(E)含量,同时观察具有理气降逆散结功效的复方中药心身5号方的调节作用。[结果]慢性情志应激可促使STZ鼠血浆NE、E含量升高,其中肾上腺素在应激+STZ组有极显著性差异(P<0.01),中药可降低血浆E含量。[结论]慢性情志刺激引起实验性2型糖尿病倾向大鼠血浆NE、E含量升高是糖尿病发病机制之一,同时具有理气降逆散结功效的复方中药心身5号方有明显调节作用。

Abstract:

[Objective] To explore the mechanisms of action of emotion stimulation in the onset of type 2 DM(Qi-ji disorder syndrome),and the effect of Xinshen NO.5,a compound prescription having effect of regulating Qi,lowering the abnormal ascending Qi and dispersing accumulation of pathogen from the pathogenesis with many factors.[Methods] The type 2 DM disease was induced by abdominal injecting a small dosage of STZ combined with psychophysiological stimulation(rotation,restraint and crowding) simultaneously.The animals were divided into five groups: normal group,stress group,STZ group,stress+STZ group and treatment group by Chinese herbs.Each group was checked for the level of serum epinephrine and norepinephrine.At the same time we observed the regulatory action of compound formula Xinshen NO.5.[Results] Emotional stimulation could increase the level of serum epinephrine and norepinephrine in STZ rats.The rats in STZ plus stimulation group had the highest content(P<0.01).Chinese herb could lower the content.[Conclusion] The increase of serum epinephrine and norepinephrine level induced by chronic emotional stimulation is one of the pathogenesis in the tendency of type 2 DM.The regulatory action of compound formula Xinshen NO.5.is apparent.