| 摘要: |
| [目的]观察益气活血复方对慢性心力衰竭大鼠心肌组织基质金属蛋白酶1(MMP-1)和基质金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响。[方法]选用雄性SD大鼠,采用冠状动脉结扎术配合饥饿、游泳等方法造成慢性心力衰竭的动物模型。将造模成功的大鼠随机分为3组:模型组、中药组和西药对照组;中药干预6周后,将动物处死,取其心肌组织,利用实时定量荧光聚合酶链反应(PCR)技术和蛋白印迹分析法(Western blot)分别检测心肌组织中MMP-1、TIMP-1基因表达和蛋白表达。[结果]与对照组比较,大鼠模型组MMP-1的表达高于正常组(P<0.01),而TIMP-1的表达明显低于正常组(P<0.01);中药组和西药组MMP-1的表达均低于模型组(P<0.01),TIMP-1的表达明显高于模型组(P<0.01)。[结论]益气活血中药可以下调MMP-1的表达,增加TIMP-1的表达,从而阻止MMP-1对心肌细胞外基质结构的破坏作用,延缓心肌重塑,改善心脏功能,从而阻止CHF的发展。 |
| 关键词: 益气活血中药 心力衰竭 MMP-1 TIMP-1 |
| DOI：10.11656/j.issn.1672-1519.2011.06.24 |
| 分类号: |
| 基金项目:十一五国家科技支撑计划项目(2007BAI20B076) |
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| Effect of traditional Chinese medicine with benefiting Qi and activating blood circulation on MMP-1 and TIMP-1 expression in rats with chronic heart failure |
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LIU Xiao-lei1, ZHANG Yan2, LI Hai1
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1.Liaoning University of TCM, Shenyang 110032, China;2.The Affiliated Hospital of Liaoning University of TCM, Shenyang 110032, China
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| Abstract: |
| [Objective] To observe the effect of the traditional Chinese medicine with benefiting qi and activating blood circulation on MMP-1 and TIMP-1 expression in rats with chronic heart failure(CHF).[Methods] We selected healthy male SD rats,established the model of heart failure by the way of coronary ligation accompanied with starvation and swimming.The rats were randomly divided into three groups: model group,Chinese medicine group,Western medicine group,and normal control group.After feeding it with drug or normal food for 6 weeks,the rats were killed and MMP-1,TIMP-1 gene expression and protein expression in myocardial tissue were detected using real-time quantitative fluorescent PCR technique and Western blot analysis(Western blot).[Results] Compared with the control group,the model group was higher than the normal group in MMP-1 expression(P<0.01),and TIMP-1 expression was significantly lower in normal group(P<0.01);the expression of MMP-1 of Chinese medicine and Western medicine group were lower than that in the model group(P<0.01),TIMP-1 expression was significantly higher than model group(P<0.01).[Conclusion] Yiqihuoxue Chinese medicine can lower the expression of MMP-1,increase the expression of TIMP-1,and thus prevent the damage of MMP-1 on the structure of the extracellular matrix function,delay myocardial remodeling and improve cardiac function,thereby prevent the development of CHF. |
| Key words: Yiqihuoxue Chinese medicine heart failure MMP-1 TIMP-1 |
