| 摘要: |
| [目的]观察补肾中药有效成分淫羊藿苷、补骨脂素、齐墩果酸对6月龄SAMP8小鼠淀粉样前体蛋白(APP)及早老素-1(PS1)基因表达的作用,为补肾中药防治老年性痴呆提供实验依据。[方法]选择6月龄健康雄性SMAP8小鼠40只,随机分为模型组、淫羊藿苷组、补骨脂素组、齐墩果酸组,每组10只。另取10只SAMR1小鼠作为对照组。治疗组分别给予淫羊藿苷、补骨脂素、齐墩果酸灌胃治疗(浓度为淫羊藿苷20 mg/kg,齐墩果酸、补骨脂素各50 mg/kg),每日1次,每次0.3 mL,连续灌胃4周。对照组与模型组均给予等量生理盐水灌胃。4周后应用逆转录-聚合酶链反应(RT-PCR)方法检测海马组织PS1基因表达。[结果]1)与对照组相比,淫羊藿苷组、齐墩果酸组及补骨脂素组对APP基因下调均具有统计学意义(P<0.05)。2)与对照组相比,补骨脂素组有下调小鼠海马组织PS1基因的趋势,淫羊藿苷组及齐墩果酸组对PS1基因下调具有统计学意义(P<0.05)。[结论]补肾中药可能通过下调APP及PS1基因的表达,进而达到治疗老年性痴呆作用。 |
| 关键词: 老年性痴呆 补肾中药 淫羊藿苷 补骨脂素 齐墩果酸 淀粉样前体蛋白基因 早老素质1基因 SAMP8鼠 |
| DOI：10.11656/j.issn.1672-1519.2012.01.20 |
| 分类号: |
| 基金项目:国家重点基础研究发展计划(973计划)项目(2010CB530405);国家自然科学基金资助项目(81102577) |
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| Clinical significance of category of cough |
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ZHANG Yu-lian1, LIU Shuang1, ZHOU Zhen1, ZHANG Lin-lin2
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1.The Second Affiliated Hospital of Tianjin University of TCM, Tianjin 300150, China;2.Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| Cough is a common clinical disease.It was traditionaly classified by traditional Chinese medicine as two categories,external affection and internal damage.This kind of definition always bring some troubles and confusion in clinical practice.is it really necessary to categorize in clinic? What are the problems that the category could solve? What are its values? These are the questions that deserve our discussion and thinking. |
| Key words: Alzheimer’s disease drug with action of reinforcing kidney APP PS1 Icariin Psoralen gene expression Oleanolic acid SAMP8 |
